EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.14 | Mg-phosphoribosyldiphosphate | requirement, phosphoribosyldiphosphate-Mg3- is the reactive molecular species of phosphoribosyldiphosphate, Km-value: 0.62 mM | Glycine max |
EC Number | General Stability | Organism |
---|---|---|
2.4.2.14 | freezing inactivates, not stabilized by 30% glycerol | Glycine max |
2.4.2.14 | high concentrations of thiol reagents stabilize during purification | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.14 | AMP | competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate | Glycine max | |
2.4.2.14 | diphosphate | uncompetitive vs. 5-phospho-alpha-D-ribose 1-diphosphate | Glycine max | |
2.4.2.14 | glutamate | competitive vs. glutamate | Glycine max | |
2.4.2.14 | GMP | - |
Glycine max | |
2.4.2.14 | IMP | - |
Glycine max | |
2.4.2.14 | NH4+ | competitive vs. glutamine | Glycine max | |
2.4.2.14 | XMP | competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.14 | additional information | - |
additional information | kinetic study | Glycine max | |
2.4.2.14 | 0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Glycine max | |
2.4.2.14 | 16 | - |
NH3 | - |
Glycine max | |
2.4.2.14 | 16 | - |
NH4+ | - |
Glycine max | |
2.4.2.14 | 18 | - |
L-glutamine | - |
Glycine max |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.14 | Mg2+ | required | Glycine max | |
2.4.2.14 | Mg2+ | 5-phospho-alpha-D-ribose 1-diphosphate-Mg3- is the reactive molecular species | Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.14 | 8000000 | - |
probably an aggregation of several purine synthetic activities, gel filtration | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.14 | L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Glycine max | enzyme of purine biosynthetic pathway, regulatory enzyme in the flow of recently fixed nitrogen from initial assimilation into amino acids via purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.14 | Glycine max | - |
soybean, Merr. cv. Williams | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.14 | Sepharose CL-2B, glycerol gradient | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.2.14 | root nodule | - |
Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.14 | 15.6 | - |
- |
Glycine max |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.14 | 4°C, 25% loss of activity per day | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.14 | L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | reaction at 50% the rate of aminotransferase activity | Glycine max | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
2.4.2.14 | L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | enzyme of purine biosynthetic pathway, regulatory enzyme in the flow of recently fixed nitrogen from initial assimilation into amino acids via purine biosynthesis | Glycine max | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
2.4.2.14 | NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | 2fold higher aminotransferase activity compared to amidotransferase activity | Glycine max | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.14 | 25 | - |
assay at | Glycine max |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.14 | 8 | - |
- |
Glycine max |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.14 | 6 | 10 | approx. 70% of maximal activity between pH 6 and pH 10 | Glycine max |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.14 | 0.65 | - |
diphosphate | - |
Glycine max | |
2.4.2.14 | 1.2 | - |
XMP | - |
Glycine max | |
2.4.2.14 | 2 | - |
AMP | - |
Glycine max | |
2.4.2.14 | 16 | - |
NH4+ | - |
Glycine max | |
2.4.2.14 | 30 | - |
glutamate | - |
Glycine max |