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Literature summary extracted from

  • Lin, L.F.H.; Lan, S.J.; Richardson, A.H.; Henderson, L.V.M.
    Pyridine nucleotide synthesis. Purification of nicotinamide mononucleotide pyrophosphorylase from rat erythrocytes (1972), J. Biol. Chem., 247, 8016-8022.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.12 EDTA 50% activation, 1 mM Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.2.12 bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.12 0.000067
-
 nicotinamide
-
 Rattus norvegicus
2.4.2.12 0.0038
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.12 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.12 Mg2+ requirement Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.12 nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate Rattus norvegicus first step in NAD synthesis from nicotinamide nicotinamide D-ribonucleotide + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.12 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.12 erythrocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.12 0.000225
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.2.12 -20°C to +20°C, about 70% loss of activity overnight Rattus norvegicus
2.4.2.12 storage at room temperature, 4°C, or frozen, unstable upon Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.12 nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus nicotinamide D-ribonucleotide + diphosphate
-
 ?
2.4.2.12 nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate first step in NAD synthesis from nicotinamide Rattus norvegicus nicotinamide D-ribonucleotide + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.12 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.12 8.5 9 assay at pH 8.8. where NAD glycohydrolase is not active Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.12 7.8 9.2 about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.12 7 10 stable Rattus norvegicus
2.4.2.12 8.8
-
 rapid inactivation below Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.2.12 ATP requirement Rattus norvegicus