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Literature summary extracted from

  • Bhatia, M.B.; Vinitsky, A.; Grubmeyer, C.
    Kinetic mechanism of orotate phosphoribosyltransferase from Salmonella typhimurium (1990), Biochemistry, 29, 10480-10487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10 structural gene pyrE, subcloned from a genomic library in pBR328(pAV002), transduced into pyr-auxotroph Salmonella typhimurium strain SA2434 with phage P22, finally cloned to and expressed in overproducing strain Escherichia coli MB13 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 5-phospho-alpha-D-ribose 1-diphosphate phosphorolysis, product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.10 diphosphate product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.10 Orotate phosphorolysis, product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.10 orotidylate product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 additional information
-
additional information
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.10 0.0031 0.0036 orotidine 5'-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.10 0.044
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 23000
-
2 * 23000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate Salmonella enterica subsp. enterica serovar Typhimurium involved in de novo synthesis of pyrimidine nucleotides orotidine 5'-phosphate + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Salmonella enterica subsp. enterica serovar Typhimurium
-
structural gene pyrE cloned and overexpressed via multi-copy plasmid in Escherichia coli strain MB13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10
-
Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.10 orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 95.7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate involved in de novo synthesis of pyrimidine nucleotides Salmonella enterica subsp. enterica serovar Typhimurium orotidine 5'-phosphate + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 dimer 2 * 23000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.10 30
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium