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Literature summary extracted from

  • Stoeckler, J.D.; Agarwal, R.P.; Agarwal, K.C.; Parks, R.E.
    Purine nucleoside phosphorylase from human erythrocytes (1978), Methods Enzymol., 51, 530-538.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Homo sapiens
2.4.2.1 PCMB
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.019
-
 hypoxanthine
-
 Homo sapiens
2.4.2.1 0.032
-
 phosphate
-
 Homo sapiens
2.4.2.1 0.047
-
 guanosine
-
 Homo sapiens
2.4.2.1 0.048
-
 Inosine
-
 Homo sapiens
2.4.2.1 0.066
-
 deoxyinosine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 30000
-
 x * 30000, SDS-PAGE Homo sapiens
2.4.2.1 80000 92000 gel filtration, equilibrium sedimentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.2.1 freezing of the enzyme in presence of dithiothreitol results in greater loss of activity than in the absence of a sulfhydryl reagent Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate ordered bi-bi mechanism with nucleoside being the first substrate to add and base product to leave Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 93
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 20°C, room temperature, stable for several days in concentrated solution, 1% in 0.02% sodium azide Homo sapiens
2.4.2.1 4°C or frozen, stable for long periods of time Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyinosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate
-
 Homo sapiens guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 ? x * 30000, SDS-PAGE Homo sapiens