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Literature summary extracted from

  • Hoffee, P.A.; May, R.; Robertson, B.C.
    Purine nucleoside phosphorylase from Salmonella typhimurium and rat liver (1978), Methods Enzymol., 51, 517-524.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.1 not stable when frozen Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.047
-
 deoxyinosine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.37
-
 phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 23500
-
 6 * 23500, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 141000
-
 equilibrium sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Rattus norvegicus
-
-
-
2.4.2.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 143
-
-
 Rattus norvegicus
2.4.2.1 160
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyinosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Rattus norvegicus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer 6 * 23500, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium