Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koops, A.J.; Jonker, H.H.
    Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Colombia. II. Purification of sucrose:sucrose 1-fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase (1996), Plant Physiol., 110, 1167-1175.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.99 pyridoxal-HCl 20 mM, stimulation to 147% of the control Helianthus tuberosus
2.4.1.99 pyridoxine 20 mM, stimulation to 120% of the control Helianthus tuberosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.99 Ag+ 1 mM, 15% inhibition Helianthus tuberosus
2.4.1.99 Cu2+ 1 mM CuSO4, 47% inhibition Helianthus tuberosus
2.4.1.99 iodoacetamide 1 mM, 10% inhibition Helianthus tuberosus
2.4.1.99 Mn2+ 5 mM 20% inhibition Helianthus tuberosus
2.4.1.99 NEM 1 mM, 10% inhibition Helianthus tuberosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.99 67000
-
 gel filtration Helianthus tuberosus
2.4.1.99 90000
-
 non-denaturing PAGE Helianthus tuberosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.99 sucrose + sucrose Helianthus tuberosus the enzyme is involved in fructan biosynthesis D-glucose + 1F-beta-D-fructosylsucrose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Helianthus tuberosus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.99 tuber
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.99 additional information
-
-
 Helianthus tuberosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 1,1-nystose + 1,1-nystose self-transfructosylation at a low rate Helianthus tuberosus 1,1,1-fructosylnystose + 1-kestose
-
 ?
2.4.1.99 1-kestose + 1-kestose self-transfructosylation at a low rate Helianthus tuberosus 1,1-nystose + sucrose
-
 ?
2.4.1.99 additional information the enzyme also catalyzes the removal of the terminal fructosyl unit from both 1-kestose and 1,1-nystose, which results in the release of sucrose and 1-kestose, respectively and free fructose. The enzyme has no hydrolytic activity against sucrose Helianthus tuberosus ?
-
 ?
2.4.1.99 sucrose + sucrose
-
 Helianthus tuberosus D-glucose + 1F-beta-D-fructosylsucrose
-
 ?
2.4.1.99 sucrose + sucrose the enzyme is involved in fructan biosynthesis Helianthus tuberosus D-glucose + 1F-beta-D-fructosylsucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.99 More electrophoresis or gel filtration under denaturing conditions yields a 27000 Da and a 55000 Da fragment Helianthus tuberosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.99 20 25 synthesis of 1-kestose Helianthus tuberosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.99 5 37 50% of maximal activity at 5°C and at 37°C, synthesis of 1-kestose Helianthus tuberosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.99 3.5 5
-
 Helianthus tuberosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.99 2.7 6.5 about 50% of maximal activity at pH 2.7 and at pH 6.5 Helianthus tuberosus