EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.255 | CDP | - |
Rattus norvegicus | |
2.4.1.255 | KCl | 50 mM causes 66% inhibition | Rattus norvegicus | |
2.4.1.255 | NaCl | 50 mM causes 66% inhibition | Rattus norvegicus | |
2.4.1.255 | thiouridine diphosphate | SUDP | Rattus norvegicus | |
2.4.1.255 | UDP | UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity | Rattus norvegicus | |
2.4.1.255 | UDP-GalNAc | UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc | Rattus norvegicus | |
2.4.1.255 | UDP-GlcNAc | UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity | Rattus norvegicus | |
2.4.1.255 | UMP | UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc | Rattus norvegicus | |
2.4.1.255 | UTP | UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.255 | 0.000545 | - |
UDP-GlcNAc | extremely high affinity | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.255 | cytosol | - |
Rattus norvegicus | 5829 | - |
2.4.1.255 | soluble | - |
Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.255 | Mg2+ | manganese choride 5 mM, essential for binding, although divalent cations are not required for activity | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.255 | 78000 | - |
2 * 110000, 1 * 78000 (alpha2beta) | Rattus norvegicus |
2.4.1.255 | 110000 | - |
2 * 110000, 1 * 78000 (alpha2beta) | Rattus norvegicus |
2.4.1.255 | 340000 | - |
on molecular sieve chromatography | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.255 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.255 | affinity chromatography, at pH 6.0-6.5 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.255 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.255 | 0.00111 | - |
last purification step | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.255 | Purified enzyme is stable and can be stored for several months at -20°C with negligible loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.255 | UDP-GlcNAc + YSDSPSTST | highly specific synthetic peptide assay, affinity of the enzyme for UDP-GlcNAc is high and for the peptide substrate, YSDSPSTST, is only moderate | Rattus norvegicus | UDP + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.255 | heterotrimer | 2 * 110000, 1 * 78000 (alpha2beta) | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.255 | O-GlcNAc transferase | - |
Rattus norvegicus |
2.4.1.255 | uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.255 | 6 | - |
about | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.255 | 6 | 7.5 | activity remaining fairly high up to pH 7.5 while dropping rapidly below 6 | Rattus norvegicus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.4.1.255 | 0.00018 | - |
- |
Rattus norvegicus | UDP-GlcNAc | |
2.4.1.255 | 0.0002 | - |
- |
Rattus norvegicus | UDP | |
2.4.1.255 | 0.0002 | - |
- |
Rattus norvegicus | UTP | |
2.4.1.255 | 0.016 | - |
- |
Rattus norvegicus | UDP-GalNAc | |
2.4.1.255 | 0.018 | - |
- |
Rattus norvegicus | UDP | |
2.4.1.255 | 0.019 | - |
- |
Rattus norvegicus | UMP |