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Literature summary extracted from

  • Sun, Y.; Hrazdina, G.
    Isolation and characterization of a UDPglucose:flavonol O3-glucosyltransferase from illuminated red cabbage (Brassica oleracea cv. Red Danish) seedlings (1991), Plant Physiol., 95, 570-576.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.007
-
 quercetin
-
 Brassica oleracea
2.4.1.91 0.012
-
 kaempferol
-
 Brassica oleracea
2.4.1.91 0.18
-
 UDPglucose
-
 Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 59000
-
 gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.91 UDPglucose + a flavonol Brassica oleracea enzyme is specific for flavonol glucoside biosynthesis UDP + a flavonol 3-O-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Brassica oleracea
-
 cv. Red Danish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91
-
 Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 seedling
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.91 additional information
-
-
 Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDPglucose + a flavonol enzyme is specific for flavonol glucoside biosynthesis Brassica oleracea UDP + a flavonol 3-O-D-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol
-
 Brassica oleracea UDP + kaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin
-
 Brassica oleracea UDP + quercetin 3-O-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.91 dimer 2 * 59000, SDS-PAGE Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 5.8 6.2
-
 Brassica oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.91 4.5 8 pH 4.5: about 80% of maximal activity, pH 8.0: about 55% of maximal activity Brassica oleracea