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Literature summary extracted from

  • Olivares-Illana, V.; Lopez-Munguia, A.; Olvera, C.
    Molecular characterization of inulosucrase from Leuconostoc citreum: a fructosyltransferase within a glucosyltransferase (2003), J. Bacteriol., 185, 3606-3612.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.9 expression of the full-length enzyme and two C-terminally truncated enzyme forms in Escherichia coli. Natural chimeric enzyme with three domains: the first and third with high identity with alternansucrase, the second domain, which is the catalytic one has similarity with levansucrase and inulosucrase Leuconostoc citreum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.9 additional information when the C-terminal domain is deleted the resulting enzyme is more active and more hydrolytic than the wild-type enzyme but is more sensitive to inactivation at 40°C Leuconostoc citreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup Leuconostoc citreum
-
 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.9 Leuconostoc citreum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
-
 Leuconostoc citreum alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup wild-type and recombinant enzyme produce inulin from sucose Leuconostoc citreum alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.9 40
-
 half-life of wild-type enzyme: 70 min, half-life of the two C-terminally truncated mutant enzymes is 20 min and 12 min Leuconostoc citreum