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Literature summary extracted from

  • Kijimoto, S.; Ishibashi, T.; Makita, A.
    Biosynthesis of Forssman hapten from globoside by alpha-N-acetylgalactosaminyltransferase of guinea pig tissues (1974), Biochem. Biophys. Res. Commun., 56, 177-184.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.88 ATP slightly activating Cavia porcellus
2.4.1.88 sodium taurocholate activation Cavia porcellus
2.4.1.88 Triton X-100 activation Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.88 ATP no inhibition Cavia porcellus
2.4.1.88 EDTA
-
 Cavia porcellus
2.4.1.88 additional information no inhibitor: NaF; not affected by GSH, N-acetylgalactosamine, and glucuronolactone Cavia porcellus
2.4.1.88 NaN3
-
 Cavia porcellus
2.4.1.88 PCMB no inhibition Cavia porcellus
2.4.1.88 sodium deoxycholate
-
 Cavia porcellus
2.4.1.88 Triton CF-54
-
 Cavia porcellus
2.4.1.88 Tween 20
-
 Cavia porcellus
2.4.1.88 Tween 80
-
 Cavia porcellus
2.4.1.88 UDP-glucose strong Cavia porcellus
2.4.1.88 UDP-N-acetylglucosamine no inhibition Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.88 0.1
-
 UDP-N-acetylgalactosamine
-
 Cavia porcellus
2.4.1.88 0.4
-
 globoside
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.88 microsome
-
 Cavia porcellus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.88 Mg2+ no effect Cavia porcellus
2.4.1.88 Mn2+ requirement Cavia porcellus
2.4.1.88 Mn2+ optimal concentration: 10 mM Cavia porcellus
2.4.1.88 Mn2+ Mg2+ and Ca2+ can hardly replace Mn2+ Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide Cavia porcellus
-
 UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.88 Cavia porcellus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.88 brain low activity Cavia porcellus
-
2.4.1.88 kidney
-
 Cavia porcellus
-
2.4.1.88 liver low activity Cavia porcellus
-
2.4.1.88 small intestine
-
 Cavia porcellus
-
2.4.1.88 spleen
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.88 additional information
-
 tissue distribution of enzyme activity Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.88 UDP-N-acetyl-alpha-D-galactosamine + globoside
-
 Cavia porcellus UDP + N-acetyl-D-galactosaminyl-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 Cavia porcellus UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.88 37
-
 assay at Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.88 6.7
-
-
 Cavia porcellus