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Literature summary extracted from

  • Reay, P.F.; Conn, E.E.
    The purification and properties of a uridine diphosphate glucose: aldehyde cyanohydrin beta-glucosyltransferase from sorghum seedlings (1974), J. Biol. Chem., 249, 5826-5830.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.85 dithiothreitol requirement, 5 mM Sorghum bicolor

General Stability

EC Number General Stability Organism
2.4.1.85 stable to freezing, DEAE-chromatography decreases stability to freezing Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.85 0.029
-
 UDPglucose
-
 Sorghum bicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.85 additional information not activated by Ca2+ or Mg2+ Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile Sorghum bicolor involved in dhurrin biosynthesis UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside dhurrin is the cyanogenic glucoside characteristic of Sorghum species ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.85 Sorghum bicolor
-
 Sudan gras hybrid, Sordan-70, cv. Moench
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.85 77fold, partial Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.85 seedling etiolated Sorghum bicolor
-
2.4.1.85 seedling shoots Sorghum bicolor
-
2.4.1.85 shoot shoots of etiolated seedlings Sorghum bicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.85 0.76
-
-
 Sorghum bicolor

Storage Stability

EC Number Storage Stability Organism
2.4.1.85 0°C, unstable at Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.85 additional information reaction is specific for an aromatic group, but not for a p-hydroxy substituent on the aromatic group, no substrates: catechol, resorcinol, phloroglucinol, fructose, glucose, dhurrin, acetone cyanohydrin, acetaldehyde cyanohydrin, GDPglucose, TDPglucose, ADPglucose Sorghum bicolor additional information no product: taxiphyllin ?
2.4.1.85 UDP-alpha-D-glucose + phenol very poor substrate Sorghum bicolor UDP + phenyl beta-D-glucopyranoside
-
 ?
2.4.1.85 UDP-glucose + 4-hydroxybenzaldehyde poor substrate, mixture of 4-hydroxybenzaldehyde with NaCN: good substrate Sorghum bicolor UDP + 4-formylphenyl beta-D-glucopyranoside
-
 ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile stereospecific for the S-enantiomer Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile involved in dhurrin biosynthesis Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside dhurrin is the cyanogenic glucoside characteristic of Sorghum species ?
2.4.1.85 UDPglucose + (S)-mandelonitrile as good as 4-hydroxymandelonitrile Sorghum bicolor UDP + (S)-mandelonitrile beta-D-glucoside
-
 ?
2.4.1.85 UDPglucose + 4-hydroxybenzoic acid poor substrate Sorghum bicolor UDP + glucosyl-4-hydroxybenzoate
-
 ?
2.4.1.85 UDPglucose + 4-hydroxybenzyl alcohol at 36% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + 4-hydroxybenzyl glucoside
-
 ?
2.4.1.85 UDPglucose + hydroquinone at 41% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + hydroquinone glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.85 30
-
 assay at Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.85 8.2 8.5
-
 Sorghum bicolor