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Literature summary extracted from

  • Paul, P.; Kamisaka, Y.; Marks, D.L.; Pagano, R.E.
    Purification and characterization of UDP-glucose:ceramide glucosyltransferase from rat liver Golgi membranes (1996), J. Biol. Chem., 271, 2287-2293.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.80 dioleoyl phosphatidylcholine or other exogenous phospholipid, required for activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.80 Golgi membrane
-
 Rattus norvegicus 139
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine Rattus norvegicus initial key enzyme in glycosphingolipid biosynthesis UDP + D-glucosyl-N-acylsphingosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.80
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.80 0.0436
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 CDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus CDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 TDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus TDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphinganine stereospecific and dependent on nature and chain length of N-acylsphinganine substrate Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine acceptor substrate specificity, overview Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine stereospecific and dependent on nature and chain length of N-acylsphingosine substrate Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine UDP-glucose is the preferred donor substrate Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine initial key enzyme in glycosphingolipid biosynthesis Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.80 ? x * 60000-66000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.80 GCS
-
 Rattus norvegicus
2.4.1.80 UDP-Glc:ceramide glucosyltransferase
-
 Rattus norvegicus