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Literature summary extracted from

  • Kamogawa, A.; Yokobayashi, K.; Fukui, T.
    Purification and properties of maltose phosphorylase from Lactobacillus brevis (1973), Agric. Biol. Chem., 37, 2813-2819.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.8 CuSO4
-
 Levilactobacillus brevis
2.4.1.8 HgCl2
-
 Levilactobacillus brevis
2.4.1.8 additional information no inhibition by MgCl2, MnSO4, CaCl2, Pb(CH3COO)2, Ba(OH)2, ZnSO4, glucose 6-phosphate, glucose 1-phosphate, fructose 6-phosphate, AMP, EDTA, IAA Levilactobacillus brevis
2.4.1.8 p-chloromercuribenzoate
-
 Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.8 1.7
-
 arsenate
-
 Levilactobacillus brevis
2.4.1.8 1.9
-
 maltose
-
 Levilactobacillus brevis
2.4.1.8 2.6
-
 phosphate
-
 Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.8 80000
-
 2 * 80000, SDS-PAGE Levilactobacillus brevis
2.4.1.8 150000
-
 gel filtration Levilactobacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.8 Levilactobacillus brevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.8
-
 Levilactobacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.8 18
-
-
 Levilactobacillus brevis

Storage Stability

EC Number Storage Stability Organism
2.4.1.8 -20°C, several months without loss of activity Levilactobacillus brevis
2.4.1.8 2°C, several months Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.8 maltose + arsenate
-
 Levilactobacillus brevis D-glucose + D-glucose + arsenate
-
 ?
2.4.1.8 maltose + phosphate
-
 Levilactobacillus brevis beta-D-glucose 1-phosphate + D-glucose
-
 r
2.4.1.8 additional information no substrate: maltitol, maltotriitol, sucrose, lactose, maltobiontic acid, maltotriose, maltotetraose Levilactobacillus brevis ?
-
 ?
2.4.1.8 additional information no substrate: trehalose, isomaltose Levilactobacillus brevis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.8 dimer 2 * 80000, SDS-PAGE Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.8 30
-
 assay at Levilactobacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.8 35
-
 30 min stable Levilactobacillus brevis
2.4.1.8 42
-
 t1/2: 30 min Levilactobacillus brevis
2.4.1.8 46
-
 t1/2: 10 min Levilactobacillus brevis
2.4.1.8 50
-
 and above, inactivation Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.8 5.4
-
-
 Levilactobacillus brevis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.8 4.5 7.2 about half-maximal activity at pH 4.5 and pH 7.2 Levilactobacillus brevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.8 5.4 7.2 stable between Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.8 additional information no pyridoxal 5'-phosphate required Levilactobacillus brevis