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Literature summary extracted from

  • Frear, D.S.
    Herbicide Metabolism in plants-I. Purification and properties of UDP-glucose:arylamine N-glucosyltransferase from soybean (1968), Phytochemistry, 7, 381-390.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.71 ammonium sulfate at high concentrations inactivates Glycine max
2.4.1.71 freeze-thawing, stable to Glycine max
2.4.1.71 stable to centrifugation at 150000 rpm for 90 min Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.71 catechol weak Glycine max
2.4.1.71 CuSO4 strong Glycine max
2.4.1.71 GSSG oxidized glutathione, weak Glycine max
2.4.1.71 HgCl2 strong Glycine max
2.4.1.71 iodoacetamide weak Glycine max
2.4.1.71 additional information no inhibition by EDTA and iodoacetate, 1 mM each Glycine max
2.4.1.71 NEM
-
 Glycine max
2.4.1.71 o-Iodosobenzoate
-
 Glycine max
2.4.1.71 p-benzoquinone strong Glycine max
2.4.1.71 PCMB strong Glycine max
2.4.1.71 Sodium arsenate 1 mM, weak Glycine max
2.4.1.71 Tris buffer
-
 Glycine max
2.4.1.71 UDP linear competitive inhibitor to UDPglucose Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.71 0.563
-
 3,4-dichloroaniline
-
 Glycine max
2.4.1.71 1.88
-
 UDPglucose
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.71 cytosol
-
 Glycine max 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.71 UDP-glucose + an arylamine Glycine max involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism UDP + an N-D-glucosylarylamine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.71 Acetone inactivates Glycine max
2.4.1.71 Ethanol inactivates Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.71 Glycine max
-
 Merril, var. Hawkeye
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.71 partial Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.71 cotyledon
-
 Glycine max
-
2.4.1.71 hypocotyl
-
 Glycine max
-
2.4.1.71 leaf
-
 Glycine max
-
2.4.1.71 root
-
 Glycine max
-
2.4.1.71 seed
-
 Glycine max
-
2.4.1.71 seedling etiolated Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.71 additional information
-
 activity in different tissues Glycine max
2.4.1.71 0.000912
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.4.1.71 -15°C, lyophilized, several months Glycine max
2.4.1.71 4°C, t1/2: 48 h Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.71 TDP-glucose + an arylamine
-
 Glycine max TDP + an N-D-glucosylarylamine
-
 ?
2.4.1.71 UDP-glucose + 2,3-dichloroaniline
-
 Glycine max UDP + N-(2,3-dichlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 2,4-dibromoaniline
-
 Glycine max UDP + N-(2,4-dibromophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 2,4-dichloroaniline
-
 Glycine max UDP + N-(2,4-dichlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 2,5-dibromoaniline
-
 Glycine max UDP + N-(2,5-dibromophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 2,5-dichloroaniline
-
 Glycine max UDP + N-(2,5-dichlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 3,4-dichloroaniline best glucosyl acceptor, no glucosyl donors are GDP-glucose, CDP-glucose, ADP-glucose, UDP-mannose Glycine max UDP + N-(3,4-dichlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 3,5-dichloroaniline
-
 Glycine max UDP + N-(3,5-dichlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 3-amino-2,5-dichlorobenzoic acid
-
 Glycine max UDP + 3-glucosylamino-2,5-dichlorobenzoic acid
-
 ?
2.4.1.71 UDP-glucose + 3-aminobenzoic acid
-
 Glycine max UDP + 3-glucosylaminobenzoic acid
-
 ?
2.4.1.71 UDP-glucose + 3-chloroaniline 31% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(3-chlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 3-nitroaniline
-
 Glycine max UDP + N-(3-nitrophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 4-bromoaniline 28% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(4-bromophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 4-chloroaniline 71% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(4-chlorophenyl)-glucosylamine
-
 ?
2.4.1.71 UDP-glucose + 5-amino-2,3-dichlorobenzoic acid
-
 Glycine max UDP + 5-glucosylamino-2,3-dichlorobenzoic acid
-
 ?
2.4.1.71 UDP-glucose + an arylamine involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism Glycine max UDP + an N-D-glucosylarylamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.71 25
-
 assay at Glycine max
2.4.1.71 28
-
 assay at Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.71 50
-
 80% loss of activity within 10 min Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.71 7.4 7.8
-
 Glycine max
2.4.1.71 7.5
-
 with 3,4-dichloroaniline as acceptor Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.71 0.484
-
 UDP
-
 Glycine max