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Literature summary extracted from

  • Karaivanova, V.; Mookerjea, S.; Hunt, D.; Nagpurkar, A.
    Characterization and purification of fucosyltransferases from the cytosol of rat colon (1996), Int. J. Biochem. Cell Biol., 28, 165-174.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 Cu2+
-
 Rattus norvegicus
2.4.1.69 NEM 20 mM, 72% inhibition Rattus norvegicus
2.4.1.152 Cu2+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.69 cytosol
-
 Rattus norvegicus 5829
-
2.4.1.69 membrane bound to Rattus norvegicus 16020
-
2.4.1.69 soluble
-
 Rattus norvegicus
-
-
2.4.1.152 membrane bound to Rattus norvegicus 16020
-
2.4.1.152 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Ca2+ soluble and membrane-bound enzyme are active in absence of added cations, but addition of 10 mM Mn2+, Mg2+ or Ca2+ stimulates Rattus norvegicus
2.4.1.69 Mg2+ soluble and membrane-bound enzyme are active in absence of added cations, but addition of 10 mM Mn2+, Mg2+ or Ca2+ stimulates Rattus norvegicus
2.4.1.69 Mn2+ soluble and membrane-bound enzyme are active in absence of added cations, but addition of 10 mM Mn2+, Mg2+ or Ca2+ stimulates Rattus norvegicus
2.4.1.152 Ca2+ Mn2+, Mg2+ or Ca2+ required Rattus norvegicus
2.4.1.152 Mg2+ Mn2+, Mg2+ or Ca2+ required Rattus norvegicus
2.4.1.152 Mn2+ Mn2+, Mg2+ or Ca2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.65 68780
-
 x * 68780, SDS-PAGE Rattus norvegicus
2.4.1.152 40680
-
 x * 40680, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Rattus norvegicus
-
-
-
2.4.1.69 Rattus norvegicus
-
-
-
2.4.1.152 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.65
-
 Rattus norvegicus
2.4.1.69
-
 Rattus norvegicus
2.4.1.152 soluble enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.65 colon
-
 Rattus norvegicus
-
2.4.1.69 colon
-
 Rattus norvegicus
-
2.4.1.69 jejunum
-
 Rattus norvegicus
-
2.4.1.152 colon
-
 Rattus norvegicus
-
2.4.1.152 jejunum
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.152 0.002289
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-L-fucose + alpha-(2,3)-sialylated Galbeta(1,3)GlcNAc
-
 Rattus norvegicus GDP + ?
-
 ?
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R
-
 Rattus norvegicus GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 GDP-L-fucose + phenyl-beta-D-galactoside
-
 Rattus norvegicus GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
 ?
2.4.1.152 GDP-L-fucose + desialylated alpha1-acid glycoprotein
-
 Rattus norvegicus GDP + ?
-
 ?
2.4.1.152 GDP-L-fucose + fetuin
-
 Rattus norvegicus GDP + ?
-
 ?
2.4.1.152 additional information 11 nonidentical amino acids, found within a hypervariable peptide segment positioned at the NH2 terminus determines whether or not an alpha(1,3)-fucosyltransferase can utilize type I acceptor substrates to form Lewis a and sialyl Lewis a moieties Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.65 ? x * 68780, SDS-PAGE Rattus norvegicus
2.4.1.152 ? x * 40680, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 5.8
-
 soluble enzyme Rattus norvegicus
2.4.1.69 6
-
 membrane-bound enzyme Rattus norvegicus
2.4.1.152 6
-
 soluble enzyme Rattus norvegicus
2.4.1.152 6.2
-
 membrane-bound enzyme Rattus norvegicus