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Literature summary extracted from

  • Masutani, H.; Kimura, H.
    Purification and characterization of secretory-type GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase from human gastric mucosa (1995), J. Biochem., 118, 541-545.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 NEM
-
 Homo sapiens
2.4.1.69 Zn2+ inhibition of plasma enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.04
-
 GDP-L-fucose enzyme from plasma Homo sapiens
2.4.1.69 0.095
-
 GDP-L-fucose enzyme from gastric mucosa Homo sapiens
2.4.1.69 0.095
-
 GDP-beta-L-fucose pH 7.2, temperature not specified in the publication Homo sapiens
2.4.1.69 0.95
-
 beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine pH 7.2, temperature not specified in the publication Homo sapiens
2.4.1.69 1.1
-
 N-acetyl-beta-lactosamine enzyme from gastric mucosa Homo sapiens
2.4.1.69 2.1
-
 N-acetyl-beta-lactosamine enzyme from plasma Homo sapiens
2.4.1.69 3.3
-
 phenyl-beta-D-galactoside enzyme from plasma Homo sapiens
2.4.1.69 4.9
-
 lactose-N-biose I enzyme from plasma Homo sapiens
2.4.1.69 13
-
 phenyl-beta-D-galactoside
-
 Homo sapiens
2.4.1.344 0.095
-
 GDP-beta-L-fucose pH 7.2, temperature not specified in the publication Homo sapiens
2.4.1.344 1.1
-
 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine pH 7.2, temperature not specified in the publication Homo sapiens
2.4.1.344 13
-
 phenyl beta-D-galactopyranoside pH 7.2, temperature not specified in the publication Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Ba2+ 10 mM, enzyme from gastric mucosa: 6.3% of the activation with Mn2+, enzyme from plasma: 108% of the activation with Mn2+ Homo sapiens
2.4.1.69 Ba2+ 10 mM, 6% of the activity with Mn2+ Homo sapiens
2.4.1.69 Ca2+ 10 mM, enzyme from gastric mucosa: 44.8% of the activation with Mn2+, enzyme from plasma: 94.2% of the activation with Mn2+ Homo sapiens
2.4.1.69 Ca2+ 10 mM, 45% of the activity with Mn2+ Homo sapiens
2.4.1.69 Co2+ 10 mM, enzyme from gastric mucosa: 48.6% of the activation with Mn2+, enzyme from plasma: 94.3% of the activation with Mn2+ Homo sapiens
2.4.1.69 Co2+ 10 mM, 48% of the activity with Mn2+ Homo sapiens
2.4.1.69 Mg2+ 10 mM, enzyme from gastric mucosa: 65.3% of the activation with Mn2+, enzyme from plasma: 98.2% of the activation with Mn2+ Homo sapiens
2.4.1.69 Mg2+ 10 mM, 65% of the activity with Mn2+ Homo sapiens
2.4.1.69 Mn2+ divalent cation is essential for enzyme from gastric mucosa, maximal activity with 10 mM Mn2+. Enzyme from plasma is maximally activated with 10 mM Mn2+, but activity is also observed without the cation Homo sapiens
2.4.1.69 Mn2+ 10 mM, best activator Homo sapiens
2.4.1.69 additional information divalent cation is absolutely required, no activation with Zn2+ Homo sapiens
2.4.1.344 Ba2+ 10 mM, 6% of the activity with Mn2+ Homo sapiens
2.4.1.344 Ca2+ 10 mM, 45% of the activity with Mn2+ Homo sapiens
2.4.1.344 Co2+ 10 mM, 48% of the activity with Mn2+ Homo sapiens
2.4.1.344 Mg2+ 10 mM, 65% of the activity with Mn2+ Homo sapiens
2.4.1.344 Mn2+ 10 mM, best activator Homo sapiens
2.4.1.344 additional information divalent cation is absolutely required, no activation with Zn2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.69 65000
-
 x * 65000, enzyme from gastric mucosa, SDS-PAGE Homo sapiens
2.4.1.69 320000
-
 gel filtration Homo sapiens
2.4.1.344 65000
-
 x * 65000, SDS-PAGE Homo sapiens
2.4.1.344 320000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Homo sapiens
-
-
-
2.4.1.344 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.69 enzyme from gastric mucosa Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.69 gastric mucosa Se enzyme Homo sapiens
-
2.4.1.69 mucosa gastric mucosa Homo sapiens
-
2.4.1.344 mucosa gastric mucosa Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.69 3.5
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose I Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine i.e. N-acetyllactosamine, reaction of EC 2.4.1.344 Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
 ?
2.4.1.69 GDP-beta-L-fucose + lactose-N-biose I
-
 Homo sapiens ?
-
 ?
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 GDP-L-fucose + lacto-N-biose 1
-
 Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
 ?
2.4.1.69 GDP-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
 ?
2.4.1.69 GDP-L-fucose + phenyl-beta-D-galactoside
-
 Homo sapiens GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
 ?
2.4.1.69 additional information enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity Homo sapiens ?
-
 ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose I Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine reaction of EC 2.4.1.69 ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine i.e. N-acetyllactosamine Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
 ?
2.4.1.344 GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
 Homo sapiens GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
 ?
2.4.1.344 additional information enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.69 ? x * 65000, enzyme from gastric mucosa, SDS-PAGE Homo sapiens
2.4.1.69 multimer x * 65000, SDS-PAGE Homo sapiens
2.4.1.344 multimer x * 65000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 7
-
 enzyme from plasma and gastric mucosa Homo sapiens