BRENDA - Enzyme Database

Purification and characterization of secretory-type GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase from human gastric mucosa

Masutani, H.; Kimura, H.; J. Biochem. 118, 541-545 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
NEM
-
Homo sapiens
2.4.1.69
Zn2+
inhibition of plasma enzyme
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
0.04
-
GDP-L-fucose
enzyme from plasma
Homo sapiens
2.4.1.69
0.095
-
GDP-beta-L-fucose
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.69
0.095
-
GDP-L-fucose
enzyme from gastric mucosa
Homo sapiens
2.4.1.69
0.95
-
beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.69
1.1
-
N-acetyl-beta-lactosamine
enzyme from gastric mucosa
Homo sapiens
2.4.1.69
2.1
-
N-acetyl-beta-lactosamine
enzyme from plasma
Homo sapiens
2.4.1.69
3.3
-
phenyl-beta-D-galactoside
enzyme from plasma
Homo sapiens
2.4.1.69
4.9
-
lactose-N-biose I
enzyme from plasma
Homo sapiens
2.4.1.69
13
-
phenyl-beta-D-galactoside
-
Homo sapiens
2.4.1.344
0.095
-
GDP-beta-L-fucose
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.344
1.1
-
beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.344
13
-
phenyl beta-D-galactopyranoside
pH 7.2, temperature not specified in the publication
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.69
Ba2+
10 mM, 6% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 6.3% of the activation with Mn2+, enzyme from plasma: 108% of the activation with Mn2+
Homo sapiens
2.4.1.69
Ca2+
10 mM, 45% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 44.8% of the activation with Mn2+, enzyme from plasma: 94.2% of the activation with Mn2+
Homo sapiens
2.4.1.69
Co2+
10 mM, 48% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 48.6% of the activation with Mn2+, enzyme from plasma: 94.3% of the activation with Mn2+
Homo sapiens
2.4.1.69
Mg2+
10 mM, 65% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 65.3% of the activation with Mn2+, enzyme from plasma: 98.2% of the activation with Mn2+
Homo sapiens
2.4.1.69
Mn2+
10 mM, best activator; divalent cation is essential for enzyme from gastric mucosa, maximal activity with 10 mM Mn2+. Enzyme from plasma is maximally activated with 10 mM Mn2+, but activity is also observed without the cation
Homo sapiens
2.4.1.69
additional information
divalent cation is absolutely required, no activation with Zn2+
Homo sapiens
2.4.1.344
Ba2+
10 mM, 6% of the activity with Mn2+
Homo sapiens
2.4.1.344
Ca2+
10 mM, 45% of the activity with Mn2+
Homo sapiens
2.4.1.344
Co2+
10 mM, 48% of the activity with Mn2+
Homo sapiens
2.4.1.344
Mg2+
10 mM, 65% of the activity with Mn2+
Homo sapiens
2.4.1.344
Mn2+
10 mM, best activator
Homo sapiens
2.4.1.344
additional information
divalent cation is absolutely required, no activation with Zn2+
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.69
65000
-
x * 65000, enzyme from gastric mucosa, SDS-PAGE
Homo sapiens
2.4.1.69
320000
-
gel filtration
Homo sapiens
2.4.1.344
65000
-
x * 65000, SDS-PAGE
Homo sapiens
2.4.1.344
320000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.69
Homo sapiens
-
-
-
2.4.1.344
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.69
enzyme from gastric mucosa
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
gastric mucosa
Se enzyme
Homo sapiens
-
2.4.1.69
mucosa
gastric mucosa
Homo sapiens
-
2.4.1.344
mucosa
gastric mucosa
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.69
3.5
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
i.e. lacto-N-biose I
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.69
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
i.e. N-acetyllactosamine, reaction of EC 2.4.1.344
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
-
489360
Homo sapiens
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
2.4.1.69
GDP-L-fucose + lacto-N-biose 1
-
489360
Homo sapiens
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + N-acetyllactosamine
-
489360
Homo sapiens
GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
-
-
?
2.4.1.69
GDP-L-fucose + phenyl-beta-D-galactoside
-
489360
Homo sapiens
GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
-
-
?
2.4.1.69
additional information
enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity
489360
Homo sapiens
?
-
-
-
-
2.4.1.344
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
i.e. lacto-N-biose I
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
reaction of EC 2.4.1.69
-
-
?
2.4.1.344
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
i.e. N-acetyllactosamine
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.344
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
489360
Homo sapiens
GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
-
-
?
2.4.1.344
additional information
enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity
489360
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.69
?
x * 65000, enzyme from gastric mucosa, SDS-PAGE
Homo sapiens
2.4.1.69
multimer
x * 65000, SDS-PAGE
Homo sapiens
2.4.1.344
multimer
x * 65000, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.69
7
-
enzyme from plasma and gastric mucosa
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
NEM
-
Homo sapiens
2.4.1.69
Zn2+
inhibition of plasma enzyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
0.04
-
GDP-L-fucose
enzyme from plasma
Homo sapiens
2.4.1.69
0.095
-
GDP-beta-L-fucose
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.69
0.095
-
GDP-L-fucose
enzyme from gastric mucosa
Homo sapiens
2.4.1.69
0.95
-
beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.69
1.1
-
N-acetyl-beta-lactosamine
enzyme from gastric mucosa
Homo sapiens
2.4.1.69
2.1
-
N-acetyl-beta-lactosamine
enzyme from plasma
Homo sapiens
2.4.1.69
3.3
-
phenyl-beta-D-galactoside
enzyme from plasma
Homo sapiens
2.4.1.69
4.9
-
lactose-N-biose I
enzyme from plasma
Homo sapiens
2.4.1.69
13
-
phenyl-beta-D-galactoside
-
Homo sapiens
2.4.1.344
0.095
-
GDP-beta-L-fucose
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.344
1.1
-
beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
pH 7.2, temperature not specified in the publication
Homo sapiens
2.4.1.344
13
-
phenyl beta-D-galactopyranoside
pH 7.2, temperature not specified in the publication
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.69
Ba2+
10 mM, 6% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 6.3% of the activation with Mn2+, enzyme from plasma: 108% of the activation with Mn2+
Homo sapiens
2.4.1.69
Ca2+
10 mM, 45% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 44.8% of the activation with Mn2+, enzyme from plasma: 94.2% of the activation with Mn2+
Homo sapiens
2.4.1.69
Co2+
10 mM, 48% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 48.6% of the activation with Mn2+, enzyme from plasma: 94.3% of the activation with Mn2+
Homo sapiens
2.4.1.69
Mg2+
10 mM, 65% of the activity with Mn2+; 10 mM, enzyme from gastric mucosa: 65.3% of the activation with Mn2+, enzyme from plasma: 98.2% of the activation with Mn2+
Homo sapiens
2.4.1.69
Mn2+
10 mM, best activator; divalent cation is essential for enzyme from gastric mucosa, maximal activity with 10 mM Mn2+. Enzyme from plasma is maximally activated with 10 mM Mn2+, but activity is also observed without the cation
Homo sapiens
2.4.1.69
additional information
divalent cation is absolutely required, no activation with Zn2+
Homo sapiens
2.4.1.344
Ba2+
10 mM, 6% of the activity with Mn2+
Homo sapiens
2.4.1.344
Ca2+
10 mM, 45% of the activity with Mn2+
Homo sapiens
2.4.1.344
Co2+
10 mM, 48% of the activity with Mn2+
Homo sapiens
2.4.1.344
Mg2+
10 mM, 65% of the activity with Mn2+
Homo sapiens
2.4.1.344
Mn2+
10 mM, best activator
Homo sapiens
2.4.1.344
additional information
divalent cation is absolutely required, no activation with Zn2+
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.69
65000
-
x * 65000, enzyme from gastric mucosa, SDS-PAGE
Homo sapiens
2.4.1.69
320000
-
gel filtration
Homo sapiens
2.4.1.344
65000
-
x * 65000, SDS-PAGE
Homo sapiens
2.4.1.344
320000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.69
enzyme from gastric mucosa
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
gastric mucosa
Se enzyme
Homo sapiens
-
2.4.1.69
mucosa
gastric mucosa
Homo sapiens
-
2.4.1.344
mucosa
gastric mucosa
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.69
3.5
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
i.e. lacto-N-biose I
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.69
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
i.e. N-acetyllactosamine, reaction of EC 2.4.1.344
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
-
489360
Homo sapiens
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
2.4.1.69
GDP-L-fucose + lacto-N-biose 1
-
489360
Homo sapiens
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + N-acetyllactosamine
-
489360
Homo sapiens
GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
-
-
?
2.4.1.69
GDP-L-fucose + phenyl-beta-D-galactoside
-
489360
Homo sapiens
GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
-
-
?
2.4.1.69
additional information
enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity
489360
Homo sapiens
?
-
-
-
-
2.4.1.344
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
i.e. lacto-N-biose I
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
reaction of EC 2.4.1.69
-
-
?
2.4.1.344
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
i.e. N-acetyllactosamine
489360
Homo sapiens
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
-
-
-
?
2.4.1.344
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
489360
Homo sapiens
GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
-
-
?
2.4.1.344
additional information
enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity
489360
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.69
?
x * 65000, enzyme from gastric mucosa, SDS-PAGE
Homo sapiens
2.4.1.69
multimer
x * 65000, SDS-PAGE
Homo sapiens
2.4.1.344
multimer
x * 65000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.69
7
-
enzyme from plasma and gastric mucosa
Homo sapiens