BRENDA - Enzyme Database

Purification of Hgene-encoded beta-galactoside alpha 1->2 fucosyltransferase from human serum

Sarnesto, A.; Koehlin, T.; Thurin, J.; Blaszczyk-Thurin, M.; J. Biol. Chem. 265, 15067-15075 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
2-mercaptoethanol
1 mM: 50% loss of activity, 10 mM: 90% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
1.6
-
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
H-gene encoded enzyme
Homo sapiens
2.4.1.69
2.5
-
Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
H-endoced and Se-encoded enzyme
Homo sapiens
2.4.1.69
2.7
-
phenyl-beta-O-galactoside
H-gene encoded enzyme
Homo sapiens
2.4.1.69
2.9
-
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
H-encoded enzyme
Homo sapiens
2.4.1.69
3.1
-
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
Se-encoded enzyme
Homo sapiens
2.4.1.69
8.4
-
phenyl-beta-O-galactoside
Se-gene encoded enzyme
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.69
50000
-
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
Homo sapiens
2.4.1.69
148000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.69
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.69
glycoprotein
-
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.69
enzyme from serum
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
blood serum
from AB and mixed secretor phenotype individuals
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.69
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
-
489349
Homo sapiens
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.69
?
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
2-mercaptoethanol
1 mM: 50% loss of activity, 10 mM: 90% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
1.6
-
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
H-gene encoded enzyme
Homo sapiens
2.4.1.69
2.5
-
Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
H-endoced and Se-encoded enzyme
Homo sapiens
2.4.1.69
2.7
-
phenyl-beta-O-galactoside
H-gene encoded enzyme
Homo sapiens
2.4.1.69
2.9
-
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
H-encoded enzyme
Homo sapiens
2.4.1.69
3.1
-
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
Se-encoded enzyme
Homo sapiens
2.4.1.69
8.4
-
phenyl-beta-O-galactoside
Se-gene encoded enzyme
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.69
50000
-
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
Homo sapiens
2.4.1.69
148000
-
gel filtration
Homo sapiens
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.69
glycoprotein
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.69
enzyme from serum
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
blood serum
from AB and mixed secretor phenotype individuals
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.69
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
-
489349
Homo sapiens
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.69
?
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
Homo sapiens