EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.69 | expression of fut1, fut2, and sec1 genes in COS-7 cells | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.69 | Ba2+ | 10 mM, complete inhibition | Sus scrofa | |
2.4.1.69 | Cd2+ | 10 mM, complete inhibition | Sus scrofa | |
2.4.1.69 | Cu2+ | 10 mM, complete inhibition | Sus scrofa | |
2.4.1.69 | GMP | competitive with GDP-L-fucose | Sus scrofa | |
2.4.1.69 | Hg2+ | 10 mM, complete inhibition | Sus scrofa | |
2.4.1.69 | lactose | competitive with antifreeze glycoprotein | Sus scrofa | |
2.4.1.69 | Ni2+ | 10 mM | Sus scrofa | |
2.4.1.69 | Zn2+ | 10 mM, complete inhibition | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.69 | 0.17 | - |
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
Sus scrofa | |
2.4.1.69 | 0.28 | - |
Galbeta(1-3)GalNAc | - |
Sus scrofa | |
2.4.1.69 | 0.63 | - |
Galbeta(1-3)GlcNAc | - |
Sus scrofa | |
2.4.1.69 | 0.94 | - |
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc | - |
Sus scrofa | |
2.4.1.69 | 1.95 | - |
Galbeta(1-6)GalNAc | - |
Sus scrofa | |
2.4.1.69 | 2.11 | - |
Galbeta(1-4)GlcNAc | - |
Sus scrofa | |
2.4.1.69 | 2.56 | - |
Galbeta(1-6)GlcNAc | - |
Sus scrofa | |
2.4.1.69 | 10.7 | - |
Galbeta1-4Glc | - |
Sus scrofa | |
2.4.1.69 | 14.6 | - |
Galbeta(1-3)Ara | - |
Sus scrofa | |
2.4.1.69 | 18 | - |
GalbetaCH3 | - |
Sus scrofa | |
2.4.1.69 | 66 | - |
GalalphaCH3 | - |
Sus scrofa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.69 | Ca2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
2.4.1.69 | Co2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
2.4.1.69 | Mg2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
2.4.1.69 | Mn2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.69 | Sus scrofa | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.69 | GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R | either a rapid equilibrium random kinetic mechanism or a steady state ordered mechanism with GDP-L-fucose binding first | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.69 | submaxillary gland | - |
Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.69 | GDP-beta-L-fucose + Galbeta(1-3)GlcNAc | - |
Sus scrofa | ? | - |
? | |
2.4.1.69 | GDP-beta-L-fucose + Galbeta(1-6)GlcNAc | - |
Sus scrofa | ? | - |
? | |
2.4.1.69 | GDP-L-fucose + beta-D-galactosyl-R | the enzyme forms the Fuc(1-2)Gal linkage with oligosaccharides, glycoproteins, and glycolipids which contain nonreducing terminal galactose residues and shows no absolute specificity for a particular penultimate residue or for the linkage between the galactose and the penultimate residue. The enzyme fucosylates human Bombay" erythrocytes which lack cell surface Fucalpha1-2Gal structures | Sus scrofa | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
2.4.1.69 | GDP-L-fucose + GalalphaCH3 | 0.1% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)GalalphaCH3 | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-3)Ara | 3.3% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-3)GalNAc | - |
Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | 84% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-4)Glc | 4.2% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-4)GlcNAc | 7.5% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc | 2.8% of the activity with Galbeta(1-3)GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-4)Man | 2.7% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)Man | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-6)GalNAc | 4.8% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc | - |
? | |
2.4.1.69 | GDP-L-fucose + GalbetaCH3 | 2.6% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)GalbetaCH3 | - |
? | |
2.4.1.69 | GDP-L-fucose + GM1 ganglioside | - |
Sus scrofa | GDP + ? | - |
? | |
2.4.1.69 | GDP-L-fucose + lacto-N-biose 1 | - |
Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc | - |
? | |
2.4.1.69 | GDP-L-fucose + lacto-N-neotetraose | - |
Sus scrofa | GDP + lacto-N-fucopentaose IV | - |
? | |
2.4.1.69 | GDP-L-fucose + paragloboside | - |
Sus scrofa | GDP + ? | - |
? |