BRENDA - Enzyme Database

Enzymatic properties of the beta-galactoside alpha 1->2 fucosyltransferase from porcine submaxillary gland

Beyer, T.A.; Hill, R.L.; J. Biol. Chem. 255, 5373-5379 (1980)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.69
expression of fut1, fut2, and sec1 genes in COS-7 cells
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
Ba2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
Cd2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
Cu2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
GMP
competitive with GDP-L-fucose
Sus scrofa
2.4.1.69
Hg2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
lactose
competitive with antifreeze glycoprotein
Sus scrofa
2.4.1.69
Ni2+
10 mM
Sus scrofa
2.4.1.69
Zn2+
10 mM, complete inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
0.17
-
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
Sus scrofa
2.4.1.69
0.28
-
Galbeta(1-3)GalNAc
-
Sus scrofa
2.4.1.69
0.63
-
Galbeta(1-3)GlcNAc
-
Sus scrofa
2.4.1.69
0.94
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc
-
Sus scrofa
2.4.1.69
1.95
-
Galbeta(1-6)GalNAc
-
Sus scrofa
2.4.1.69
2.11
-
Galbeta(1-4)GlcNAc
-
Sus scrofa
2.4.1.69
2.56
-
Galbeta(1-6)GlcNAc
-
Sus scrofa
2.4.1.69
10.7
-
Galbeta1-4Glc
-
Sus scrofa
2.4.1.69
14.6
-
Galbeta(1-3)Ara
-
Sus scrofa
2.4.1.69
18
-
GalbetaCH3
-
Sus scrofa
2.4.1.69
66
-
GalalphaCH3
-
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.69
Ca2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Co2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Mg2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Mn2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.69
Sus scrofa
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.4.1.69
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R
either a rapid equilibrium random kinetic mechanism or a steady state ordered mechanism with GDP-L-fucose binding first
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
submaxillary gland
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
the enzyme forms the Fuc(1-2)Gal linkage with oligosaccharides, glycoproteins, and glycolipids which contain nonreducing terminal galactose residues and shows no absolute specificity for a particular penultimate residue or for the linkage between the galactose and the penultimate residue. The enzyme fucosylates human Bombay" erythrocytes which lack cell surface Fucalpha1-2Gal structures
489343
Sus scrofa
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
2.4.1.69
GDP-L-fucose + GalalphaCH3
0.1% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)GalalphaCH3
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)Ara
3.3% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)GalNAc
-
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
84% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)Glc
4.2% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)GlcNAc
7.5% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc
2.8% of the activity with Galbeta(1-3)GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)Man
2.7% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)Man
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-6)GalNAc
4.8% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + GalbetaCH3
2.6% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)GalbetaCH3
-
-
-
?
2.4.1.69
GDP-L-fucose + GM1 ganglioside
-
489343
Sus scrofa
GDP + ?
-
489343
Sus scrofa
?
2.4.1.69
GDP-L-fucose + lacto-N-biose 1
-
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + lacto-N-neotetraose
-
489343
Sus scrofa
GDP + lacto-N-fucopentaose IV
-
-
-
-
2.4.1.69
GDP-L-fucose + paragloboside
-
489343
Sus scrofa
GDP + ?
-
489343
Sus scrofa
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.69
expression of fut1, fut2, and sec1 genes in COS-7 cells
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.69
Ba2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
Cd2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
Cu2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
GMP
competitive with GDP-L-fucose
Sus scrofa
2.4.1.69
Hg2+
10 mM, complete inhibition
Sus scrofa
2.4.1.69
lactose
competitive with antifreeze glycoprotein
Sus scrofa
2.4.1.69
Ni2+
10 mM
Sus scrofa
2.4.1.69
Zn2+
10 mM, complete inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.69
0.17
-
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
Sus scrofa
2.4.1.69
0.28
-
Galbeta(1-3)GalNAc
-
Sus scrofa
2.4.1.69
0.63
-
Galbeta(1-3)GlcNAc
-
Sus scrofa
2.4.1.69
0.94
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc
-
Sus scrofa
2.4.1.69
1.95
-
Galbeta(1-6)GalNAc
-
Sus scrofa
2.4.1.69
2.11
-
Galbeta(1-4)GlcNAc
-
Sus scrofa
2.4.1.69
2.56
-
Galbeta(1-6)GlcNAc
-
Sus scrofa
2.4.1.69
10.7
-
Galbeta1-4Glc
-
Sus scrofa
2.4.1.69
14.6
-
Galbeta(1-3)Ara
-
Sus scrofa
2.4.1.69
18
-
GalbetaCH3
-
Sus scrofa
2.4.1.69
66
-
GalalphaCH3
-
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.69
Ca2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Co2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Mg2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
2.4.1.69
Mn2+
the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.69
submaxillary gland
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.69
GDP-L-fucose + beta-D-galactosyl-R
the enzyme forms the Fuc(1-2)Gal linkage with oligosaccharides, glycoproteins, and glycolipids which contain nonreducing terminal galactose residues and shows no absolute specificity for a particular penultimate residue or for the linkage between the galactose and the penultimate residue. The enzyme fucosylates human Bombay" erythrocytes which lack cell surface Fucalpha1-2Gal structures
489343
Sus scrofa
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
-
-
?
2.4.1.69
GDP-L-fucose + GalalphaCH3
0.1% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)GalalphaCH3
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)Ara
3.3% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)GalNAc
-
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
84% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)Glc
4.2% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)GlcNAc
7.5% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc
2.8% of the activity with Galbeta(1-3)GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-4)Man
2.7% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-4)Man
-
-
-
?
2.4.1.69
GDP-L-fucose + Galbeta(1-6)GalNAc
4.8% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + GalbetaCH3
2.6% of the activity with Galbeta1-3GalNAc
489343
Sus scrofa
GDP + L-Fucalpha(1-2)GalbetaCH3
-
-
-
?
2.4.1.69
GDP-L-fucose + GM1 ganglioside
-
489343
Sus scrofa
GDP + ?
-
489343
Sus scrofa
?
2.4.1.69
GDP-L-fucose + lacto-N-biose 1
-
489343
Sus scrofa
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
-
-
?
2.4.1.69
GDP-L-fucose + lacto-N-neotetraose
-
489343
Sus scrofa
GDP + lacto-N-fucopentaose IV
-
-
-
-
2.4.1.69
GDP-L-fucose + paragloboside
-
489343
Sus scrofa
GDP + ?
-
489343
Sus scrofa
?