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Literature summary extracted from

  • Beyer, T.A.; Hill, R.L.
    Enzymatic properties of the beta-galactoside alpha 1->2 fucosyltransferase from porcine submaxillary gland (1980), J. Biol. Chem., 255, 5373-5379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 expression of fut1, fut2, and sec1 genes in COS-7 cells Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 Ba2+ 10 mM, complete inhibition Sus scrofa
2.4.1.69 Cd2+ 10 mM, complete inhibition Sus scrofa
2.4.1.69 Cu2+ 10 mM, complete inhibition Sus scrofa
2.4.1.69 GMP competitive with GDP-L-fucose Sus scrofa
2.4.1.69 Hg2+ 10 mM, complete inhibition Sus scrofa
2.4.1.69 lactose competitive with antifreeze glycoprotein Sus scrofa
2.4.1.69 Ni2+ 10 mM Sus scrofa
2.4.1.69 Zn2+ 10 mM, complete inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.17
-
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
Sus scrofa
2.4.1.69 0.28
-
Galbeta(1-3)GalNAc
-
Sus scrofa
2.4.1.69 0.63
-
Galbeta(1-3)GlcNAc
-
Sus scrofa
2.4.1.69 0.94
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc
-
Sus scrofa
2.4.1.69 1.95
-
Galbeta(1-6)GalNAc
-
Sus scrofa
2.4.1.69 2.11
-
Galbeta(1-4)GlcNAc
-
Sus scrofa
2.4.1.69 2.56
-
Galbeta(1-6)GlcNAc
-
Sus scrofa
2.4.1.69 10.7
-
Galbeta1-4Glc
-
Sus scrofa
2.4.1.69 14.6
-
Galbeta(1-3)Ara
-
Sus scrofa
2.4.1.69 18
-
GalbetaCH3
-
Sus scrofa
2.4.1.69 66
-
GalalphaCH3
-
Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Ca2+ the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ Sus scrofa
2.4.1.69 Co2+ the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ Sus scrofa
2.4.1.69 Mg2+ the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ Sus scrofa
2.4.1.69 Mn2+ the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Sus scrofa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R either a rapid equilibrium random kinetic mechanism or a steady state ordered mechanism with GDP-L-fucose binding first Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.69 submaxillary gland
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + Galbeta(1-3)GlcNAc
-
Sus scrofa ?
-
?
2.4.1.69 GDP-beta-L-fucose + Galbeta(1-6)GlcNAc
-
Sus scrofa ?
-
?
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R the enzyme forms the Fuc(1-2)Gal linkage with oligosaccharides, glycoproteins, and glycolipids which contain nonreducing terminal galactose residues and shows no absolute specificity for a particular penultimate residue or for the linkage between the galactose and the penultimate residue. The enzyme fucosylates human Bombay" erythrocytes which lack cell surface Fucalpha1-2Gal structures Sus scrofa GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
?
2.4.1.69 GDP-L-fucose + GalalphaCH3 0.1% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)GalalphaCH3
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-3)Ara 3.3% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-3)GalNAc
-
Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc 84% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-4)Glc 4.2% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-4)GlcNAc 7.5% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc 2.8% of the activity with Galbeta(1-3)GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-4)Man 2.7% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-4)Man
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1-6)GalNAc 4.8% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
-
?
2.4.1.69 GDP-L-fucose + GalbetaCH3 2.6% of the activity with Galbeta1-3GalNAc Sus scrofa GDP + L-Fucalpha(1-2)GalbetaCH3
-
?
2.4.1.69 GDP-L-fucose + GM1 ganglioside
-
Sus scrofa GDP + ?
-
?
2.4.1.69 GDP-L-fucose + lacto-N-biose 1
-
Sus scrofa GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
?
2.4.1.69 GDP-L-fucose + lacto-N-neotetraose
-
Sus scrofa GDP + lacto-N-fucopentaose IV
-
?
2.4.1.69 GDP-L-fucose + paragloboside
-
Sus scrofa GDP + ?
-
?