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Literature summary extracted from

  • Risteli, L.
    Further characterization of collagen galactosyltransferase from chick embryos (1978), Biochem. J., 169, 189-196.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.50 dithiothreitol 20 mM, activates Gallus gallus
2.4.1.50 Nonidet P-40 activates Gallus gallus
2.4.1.50 Triton X-100 0.1%, 2fold stimulation Gallus gallus
2.4.1.50 Urea 2 mM, activates Gallus gallus

General Stability

EC Number General Stability Organism
2.4.1.50 highly labile enzyme, DTT stabilizes during purification, 0.25 M or 0.1 M galactose or glycerol 25% v/v do not stabilize Gallus gallus
2.4.1.50 unstable during purification Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.50 ADP
-
Gallus gallus
2.4.1.50 AMP
-
Gallus gallus
2.4.1.50 ATP
-
Gallus gallus
2.4.1.50 CDP
-
Gallus gallus
2.4.1.50 Co2+ 6 mM: 50% inhibition Gallus gallus
2.4.1.50 concanavalin A 0.3 M methyl-alpha-D-glucopyranoside protects, but not galactose Gallus gallus
2.4.1.50 Galactosylhydroxylysine
-
Gallus gallus
2.4.1.50 GDP
-
Gallus gallus
2.4.1.50 gelatinized calf skin collagen above 60 mg/ml Gallus gallus
2.4.1.50 human renal basement membrane collagen glucose-free Gallus gallus
2.4.1.50 hydroxylysine weak Gallus gallus
2.4.1.50 Mn2+ above 2.5 mM Gallus gallus
2.4.1.50 additional information not inhibited by lysine, free monosaccharides, 1 mM lactose, 1 mM sucrose, 2 mM uridine Gallus gallus
2.4.1.50 UDP
-
Gallus gallus
2.4.1.50 UDPgalacturonate
-
Gallus gallus
2.4.1.50 UDPglucose
-
Gallus gallus
2.4.1.50 UDPglucuronate
-
Gallus gallus
2.4.1.50 UMP
-
Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.50 additional information
-
additional information Km: 16 mg/ml, gelatinized calf skin collagen Gallus gallus
2.4.1.50 0.02 0.023 UDPgalactose
-
Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.50 endoplasmic reticulum cisterna presumed location within Gallus gallus
-
-
2.4.1.50 membrane associated with membrane structures Gallus gallus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.50 Mn2+ requirement Gallus gallus
2.4.1.50 Mn2+ optimal concentration: 2-2.5 mM Gallus gallus
2.4.1.50 additional information not activated by Co2+ Gallus gallus
2.4.1.50 additional information not activated by Mg2+ Gallus gallus
2.4.1.50 additional information not activated by Fe2+ Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.50 additional information Gallus gallus collagen biosynthesis ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.50 Ethylene glycol 50% v/v, stable to Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.50 Gallus gallus
-
white Leghorn chicken
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.50 glycoprotein presence of carbohydrate units in enzyme molecule Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.50 250-300fold Gallus gallus
2.4.1.50 optimal extraction requires high concentrations of detergent and salt Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.50 embryo 14-15 days old Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.50 -20°C, 50% v/v ethylene glycol, several weeks, stable Gallus gallus
2.4.1.50 4°C, unstable to storage, urea, galactose, Triton X-100, glycerol or ethylene glycol do not stabilize Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.50 additional information no acceptor: free hydroxylysine Gallus gallus ?
-
?
2.4.1.50 additional information collagen biosynthesis Gallus gallus ?
-
?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.50 37
-
assay at Gallus gallus