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Literature summary extracted from

  • Takeuchi, M.; Yoshikawa, M.; Sasaki, R.; Chiba, R.
    Purification and characterization of UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase from mammary gland of lacting cow (1985), Agric. Biol. Chem., 49, 1059-1069.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.41 DTT required for maximal activity Bos taurus
2.4.1.41 Triton X-100 required for maximal activity Bos taurus

Application

EC Number Application Comment Organism
2.4.1.41 molecular biology enzyme will be useful for the in vitro glycosylation of proteins obtained from microorganisms by gene manipulation techniques Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 ADP weak Bos taurus
2.4.1.41 alphaS1-casein strong inhibition of GalNAc transfer to kappa-casein, reversed by Ca2+ Bos taurus
2.4.1.41 AMP
-
 Bos taurus
2.4.1.41 ATP weak Bos taurus
2.4.1.41 CDP
-
 Bos taurus
2.4.1.41 CMP
-
 Bos taurus
2.4.1.41 CTP
-
 Bos taurus
2.4.1.41 GDP weak Bos taurus
2.4.1.41 GMP
-
 Bos taurus
2.4.1.41 GTP
-
 Bos taurus
2.4.1.41 Helix pomatia lectin
-
 Bos taurus
2.4.1.41 additional information not inhibited by wheat germ lectin Bos taurus
2.4.1.41 Soybean lectin
-
 Bos taurus
2.4.1.41 UDP strong Bos taurus
2.4.1.41 UMP
-
 Bos taurus
2.4.1.41 UTP
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 additional information
-
 additional information Km: 0.192 mg/ml: apomucin, 1.15 mg/ml: kappa-subcomponent 1, 5.1 mg/ml: kappa-subcomponent 7 Bos taurus
2.4.1.41 0.0162
-
 UDP-GalNAc
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 Golgi membrane specifically localized in Bos taurus 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Ba2+ stimulates to a smaller extent than Mn2+ Bos taurus
2.4.1.41 Ca2+ stimulates to a smaller extent than Mn2+ Bos taurus
2.4.1.41 Mg2+ stimulates to a smaller extent than Mn2+ Bos taurus
2.4.1.41 Mn2+ requirement Bos taurus
2.4.1.41 Mn2+ strong stimulation, maximal activity at 4-10 mM Bos taurus
2.4.1.41 Zn2+ stimulates to a smaller extent than Mn2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 200000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + kappa-casein Bos taurus glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Bos taurus
-
 lactating cow
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 7500fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 mammary gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 0.00863
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information not: alphaS1-casein, alphaS2-casein, beta-casein, alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin Bos taurus ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apomucin best substrate, poorer substrates: asialo mucin, native mucin Bos taurus UDP + N-acetyl-D-galactosaminyl-apomucin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + kappa-casein kappa-subcomponent 1, the nonglycosylated kappa-casein, is the best substrate among kappa-casein subcomponents, asialo kappa-casein, agalacto kappa-casein and nature kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + kappa-casein glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo Bos taurus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptor: asialo mucin, subcomponents of kappa-casein, asialo kappa-casein, agalacto kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: apomucin, kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 25
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.41 4 35 1 h, less than 20% loss of activity Bos taurus
2.4.1.41 45
-
 1 h, 50% loss of activity Bos taurus
2.4.1.41 55
-
 1 h, complete loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.41 additional information
-
 pI: 6.4 Bos taurus
2.4.1.41 6.5 7.5 Tris, imidazole and MES buffer, kappa-casein Bos taurus