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Literature summary extracted from

  • Mulichak, A.M.; Losey, H.C.; Walsh, C.T.; Garavito, R.M.
    Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics (2001), Structure, 9, 547-557.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.34 two distinct N- and C-terminal halves, similar in size and topology and separated by a deep cleft, both domains contain a similar core structure of parallel beta sheets connected by alpha helices, the C-terminal structure contains a glycine fingerprint sequence Staphylococcus aureus
2.4.1.310 hanging drop vapor diffusion method, 1.8 resolution Amycolatopsis orientalis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.34 D13A lower turnover and lower Km than wild-type Staphylococcus aureus
2.4.1.34 D332A lower turnover and higher Km than wild-type Staphylococcus aureus
2.4.1.34 H125A higher turnover and lower Km than wild-type Staphylococcus aureus
2.4.1.310 D13A kcat/Km is 9.2% compared to the wild-type value Amycolatopsis orientalis
2.4.1.310 D332A kcat/Km is 0.16% compared to the wild-type value Amycolatopsis orientalis
2.4.1.310 H125A kcat/Km is 3.8fold higher than the wild-type value Amycolatopsis orientalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.34 0.45
-
 UDPglucose H125A Staphylococcus aureus
2.4.1.34 1.1
-
 UDPglucose mutant D13A Staphylococcus aureus
2.4.1.34 1.3
-
 UDPglucose wild-type Staphylococcus aureus
2.4.1.34 3.3
-
 UDPglucose mutant D332A Staphylococcus aureus
2.4.1.310 0.45
-
 UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 1.1
-
 UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 1.3
-
 UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 3.3
-
 UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin UDP + devancoaminyl vancomycin
-
 ?
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis A82846 the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin UDP + devancoaminyl vancomycin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.34 Staphylococcus aureus
-
-
-
2.4.1.310 Amycolatopsis orientalis P96559
-
-
2.4.1.310 Amycolatopsis orientalis A82846 P96559
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.310
-
 Amycolatopsis orientalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Staphylococcus aureus UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
 ?
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
 ?
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
 ?
2.4.1.310 UDP-alpha-D-glucose + vancomycin aglycone the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.310 GtfB
-
 Amycolatopsis orientalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.34 0.00217
-
 UDPglucose D332A Staphylococcus aureus
2.4.1.34 0.0433
-
 UDPglucose D13A Staphylococcus aureus
2.4.1.34 0.55
-
 UDPglucose wild-type Staphylococcus aureus
2.4.1.34 0.717
-
 UDPglucose H125A Staphylococcus aureus
2.4.1.310 0.0022
-
 UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 0.043
-
 UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 0.55
-
 UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 0.72
-
 UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.310 0.00067
-
 UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 0.039
-
 UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 0.42
-
 UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
2.4.1.310 1.6
-
 UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis