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Literature summary extracted from

  • Goldemberg, S.H.; Marechal, L.R.; De Souza, B.C.
    beta-1,3-Oligoglucan: orthophosphate glucosyltransferase from Euglena gracilis (1966), J. Biol. Chem., 241, 45-50.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.31 additional information not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate Euglena gracilis

General Stability

EC Number General Stability Organism
2.4.1.31 stable to repeated freezing and thawing Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.31 2-deoxyglucose 25-30% inhibition at 20 mM Euglena gracilis
2.4.1.31 D-fructose 25-30% inhibition at 20 mM Euglena gracilis
2.4.1.31 D-glucosamine 25-30% inhibition at 20 mM Euglena gracilis
2.4.1.31 D-glucose 25-30% inhibition at 10 mM Euglena gracilis
2.4.1.31 D-mannose 25-30% inhibition at 20 mM Euglena gracilis
2.4.1.31 diphosphate 10% inhibition at 1 mM, pH 7 Euglena gracilis
2.4.1.31 fructose 6-phosphate 10% inhibition at 1 mM Euglena gracilis
2.4.1.31 HgCl2 10% inhibition at 1 mM Euglena gracilis
2.4.1.31 imidazole weak, 25% inhibition at 0.4 M Euglena gracilis
2.4.1.31 additional information not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate Euglena gracilis
2.4.1.31 NADP+ 10% inhibition at 1 mM Euglena gracilis
2.4.1.31 p-hydroxymercuribenzoate 40% inhibition at 0.2 mM and 80% inhibition at 2 mM Euglena gracilis
2.4.1.31 phloridzin 10% inhibition at 1 mM Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.31 additional information
-
 additional information linear within certain limits Euglena gracilis
2.4.1.31 2.1
-
 alpha-D-glucose 1-phosphate with glucose addition Euglena gracilis
2.4.1.31 2.5
-
 phosphate with laminaribiose addition Euglena gracilis
2.4.1.31 5
-
 laminaribiose with phosphate addition Euglena gracilis
2.4.1.31 6
-
 laminaritriose with phosphate addition Euglena gracilis
2.4.1.31 19
-
 D-glucose with glucose 1-phosphate addition Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.31 protamine sulfate and ammonium sulfate precipitation, calcium phosphate gel adsorption and DEAE-cellulose chromatography Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.31 24
-
 D-glucose + D-glucose 1-phosphate, in DEAE-eluate Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.4.1.31 -14°C, 3 months stable Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + arbutin
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + cellobiose
-
 Euglena gracilis phosphate + oligosaccharides
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose specific for alpha-D-glucose 1-phosphate as glucosyl donor Euglena gracilis laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate amount of individual products depends on substrate concentration and reaction time ?
2.4.1.31 alpha-D-glucose 1-phosphate + laminaribiose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaritetraose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaritriose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
 Euglena gracilis ?
-
 ?
2.4.1.31 alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + salicin
-
 Euglena gracilis ?
-
 r
2.4.1.31 D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis 3-beta-D-glucosyl-D-glucose + phosphate
-
 ?
2.4.1.31 laminaripentaose + phosphate
-
 Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 laminaritetraose + phosphate
-
 Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 additional information arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate Euglena gracilis ?
-
 ?
2.4.1.31 additional information weak or no activity observed with other carbohydrates Euglena gracilis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.31 37
-
 assay at Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.31 6.3 6.9 same optimum pH for for forward and reverse reaction Euglena gracilis
2.4.1.31 6.5
-
 assay at Euglena gracilis