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Literature summary extracted from

  • Roujeinikova, A.; Raasch, C.; Sedelnikova, S.; Liebl, W.; Rice, D.W.
    Crystal structure of Thermotoga maritima 4-alpha-glucanotransferase and its acarbose complex: Implications for substrate specificity and catalysis (2002), J. Mol. Biol., 321, 149-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 cloned and expressed in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.25
-
Thermus aquaticus
2.4.1.25 Gtase, hanging drop method, inhibitor complex,crystals belong to space group I222, unit-cell dimensions a = 92.6 A, b = 180.3 A, c = 199.2 A, free Gtase crystals a = 94.5 A, b = 181.4 A, c = 197.3 A Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Aquifex aeolicus
-
-
-
2.4.1.25 Clostridium butyricum
-
-
-
2.4.1.25 Dictyoglomus thermophilum
-
-
-
2.4.1.25 Escherichia coli
-
-
-
2.4.1.25 Haemophilus influenzae
-
-
-
2.4.1.25 Thermococcus litoralis
-
-
-
2.4.1.25 Thermotoga maritima
-
-
-
2.4.1.25 Thermotoga neapolitana
-
-
-
2.4.1.25 Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylopectin + maltose Gtase Thermotoga maritima ?
-
?
2.4.1.25 amylose + maltose Gtase Thermotoga maritima ?
-
?
2.4.1.25 maltotriose + maltotriose
-
Thermococcus litoralis maltodextrins
-
?
2.4.1.25 maltotriose + maltotriose Mtase Thermotoga maritima maltodextrins
-
?
2.4.1.25 additional information
-
Escherichia coli ?
-
?
2.4.1.25 additional information
-
Thermococcus litoralis ?
-
?
2.4.1.25 additional information 2 different 4-alpha-glucanotransferases, MTAse and Gtase Thermotoga maritima ?
-
?
2.4.1.25 additional information Gtase, glucose does not function as acceptor sugar, nor does it appear as reaction product Thermotoga maritima ?
-
?
2.4.1.25 starch + maltose Gtase Thermotoga maritima ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 GTase
-
Thermotoga maritima