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Literature summary extracted from

  • Van der Maarel, M.J.E.C.; Euverink, G.J.W.; Binnema, D.J.; Bos, H.T.P.; Bergsma, J.
    Amylomaltase from the hyperthermophilic bacterium Thermus thermophilus: Enzyme characteristics and application in the starch industry (2000), Meded. -Fac. Landbouwkd. Toegepaste Biol. Wet., 65, 231-234.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.25 nutrition potential applications in the starch industry Thermus thermophilus
2.4.1.25 synthesis potential applications in the starch industry Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 malQ gene clone in pET expression vector system Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 12
-
maltotriose
-
Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 56000
-
-
Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Solanum tuberosum
-
potato
-
2.4.1.25 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
Thermus thermophilus maltooligosaccharides
-
?
2.4.1.25 1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
Solanum tuberosum maltooligosaccharides
-
?
2.4.1.25 1,4-alpha-D-glucan + glucose
-
Thermus thermophilus maltooligosaccharides
-
?
2.4.1.25 1,4-alpha-D-glucan + glucose
-
Solanum tuberosum maltooligosaccharides
-
?
2.4.1.25 maltotriose + maltotriose
-
Thermus thermophilus maltopentaose + maltotetraose
-
?
2.4.1.25 additional information glucose can only be used as acceptor of maltosyl units Thermus thermophilus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 monomer
-
Thermus thermophilus
2.4.1.25 monomer
-
Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 68
-
-
Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6.2
-
-
Thermus thermophilus