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Literature summary extracted from

  • Liebl, W.; Feil, R.; Gabelsberger, J.; Kellermann, J.; Schleifer, K.H.
    Purification and characterization of a novel thermostable 4-alpha-glucanotransferase of Thermotoga maritima cloned in Escherichia coli (1992), Eur. J. Biochem., 207, 81-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.25 maltoheptaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 124% Thermotoga maritima
2.4.1.25 maltohexaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 144% Thermotoga maritima
2.4.1.25 maltopentaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima
2.4.1.25 maltose stimulating effect of maltooligosaccharides on the conversion of amylose, relative activity 259% Thermotoga maritima
2.4.1.25 maltotetraose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 246% Thermotoga maritima
2.4.1.25 maltotriose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 chromosomal gene cloned and expressed in Escherichia coli and Corynebacterium glutamicum Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.25 cytoplasm
-
Thermotoga maritima 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 53000
-
SDS-PAGE Thermotoga maritima
2.4.1.25 56000
-
size exclusion chromatography Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.25 maltopentaose + maltopentaose Bacillus sp. (in: Bacteria) metabolism of starch in the bacterium maltooligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose Thermotoga maritima metabolism of starch in the bacterium maltooligosaccharides
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Bacillus sp. (in: Bacteria)
-
-
-
2.4.1.25 Thermotoga maritima
-
MSB8
-
2.4.1.25 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
MSB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylopectin + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 amylose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 maltoheptaose + maltopentaose
-
Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
2.4.1.25 maltoheptaose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 maltohexaose + maltopentaose
-
Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
2.4.1.25 maltohexaose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose
-
Bacillus sp. (in: Bacteria) new oligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose
-
Thermotoga maritima new oligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 new oligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
?
2.4.1.25 additional information maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product Thermotoga maritima ?
-
?
2.4.1.25 additional information does not use maltose, maltotriose or maltotetraose as acceptor substrates in maltodextrinyltransfer reactions Bacillus sp. (in: Bacteria) ?
-
?
2.4.1.25 additional information alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose Thermotoga maritima ?
-
?
2.4.1.25 additional information maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
2.4.1.25 additional information alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
2.4.1.25 starch + maltopentaose
-
Bacillus sp. (in: Bacteria) alpha-1,4-D-glucans
-
?
2.4.1.25 starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima alpha-1,4-D-glucans
-
?
2.4.1.25 starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-1,4-D-glucans
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 monomer 1 * 53000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 GTase
-
Thermotoga maritima
2.4.1.25 maltodextrin glycosyltransferase
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 70
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.25 30 90
-
Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 37 80 stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 7 8
-
Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.25 3.5 10
-
Thermotoga maritima