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Literature summary extracted from

  • Wei, G.; Bai, X.; Gabb, M.M.G.; Bame, K.J.; Koshy, T.I.; Spear, P.G.; Esko, J.D.
    Location of the glucuronosyltransferase domain in the heparan sulfate copolymerase EXT1 by analysis of Chinese hamster ovary cell mutants (2000), J. Biol. Chem., 275, 27733-27740.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.224 detergent required, range from 0.03-1% Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.224 EXT1 and EXT2 from wild type CHO used to complement deficiency mutant CHO-K1, EXT2 failed to complement functional deficiency while it was fully corrected by EXT1 Mus musculus
2.4.1.225 expression in CHO cells Oncorhynchus mykiss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.224 0.3
-
 UDP-N-acetyl-D-glucosamine
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.224 Mn2+ required, can not be substituted by other divalent cations Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Mus musculus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Homo sapiens elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Cricetinae elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.225 UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan Oncorhynchus mykiss
-
 UDP + beta-glucuronosyl-(1-4)-N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.224 Cricetinae
-
 mutation in pgsD gene locus
-
2.4.1.224 Cricetinae
-
 deficiency mutant CHO-K1 used for experiments
-
2.4.1.224 Homo sapiens
-
-
-
2.4.1.224 Mus musculus
-
-
-
2.4.1.225 Oncorhynchus mykiss
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.225 ovary
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.224 additional information
-
 specific activities of different pgsD mutants tested Mus musculus
2.4.1.225 0.000012
-
-
 Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Cricetinae UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Cricetinae UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.225 UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 Oncorhynchus mykiss UDP + beta-glucuronosyl-(1-4)-N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.224 5 8
-
 Mus musculus