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Literature summary extracted from

  • Ruyter, C.M.; Stoeckigt, J.H.H.
    Enzymatic formation of Raucaffricine, the major indole alkaloid of Rauwolfia serpentina cell-suspension cultures (1991), Helv. Chim. Acta, 74, 1707-1712.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.219 2-mercaptoethanol stabilizes transferase activity Rauvolfia serpentina
2.4.1.219 ascorbic acid stabilizes transferase activity Rauvolfia serpentina
2.4.1.219 dithioerythritol stabilizes transferase activity Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.219 (phenylmethyl)sulfonyl fluoride strong inhibition at 10 mM Rauvolfia serpentina
2.4.1.219 1,10-phenanthroline strong inhibition at 5 mM Rauvolfia serpentina
2.4.1.219 5,5'-dithiobis(2-nitrobenzoic acid) strong inhibition at 1.25 mM Rauvolfia serpentina
2.4.1.219 Cu2+ complete inhibition at 10 mM Rauvolfia serpentina
2.4.1.219 EDTA strong inhibition at 5 mM Rauvolfia serpentina
2.4.1.219 Fe3+ complete inhibition at 10 mM Rauvolfia serpentina
2.4.1.219 Hg2+ complete inhibition at 10 mM Rauvolfia serpentina
2.4.1.219 p-Hydroxybenzaldehyde strong inhibition at 5 mM Rauvolfia serpentina
2.4.1.219 Zn2+ complete inhibition at 10 mM Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.219 0.04
-
 Vomilenine
-
 Rauvolfia serpentina
2.4.1.219 0.8
-
 UDP-glucose
-
 Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.219 UDP-glucose + vomilenine Rauvolfia serpentina
-
 UDP + raucaffricine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.219 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.219
-
 Rauvolfia serpentina

Storage Stability

EC Number Storage Stability Organism
2.4.1.219 -20°C, 3 weeks, no loss in activity Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.219 UDP-glucose + vomilenine
-
 Rauvolfia serpentina UDP + raucaffricine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.219 50
-
-
 Rauvolfia serpentina