EC Number | General Stability | Organism |
---|---|---|
2.4.1.189 | freezing without glycerol, complete inactivation | Secale cereale |
2.4.1.190 | freezing without glycerol, complete inactivation | Secale cereale |
2.4.1.191 | freezing without glycerol, complete loss of activity | Secale cereale |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.189 | luteolin | 0.14 mM, 60% inhibition | Secale cereale | |
2.4.1.189 | UDP | strong inhibition | Secale cereale | |
2.4.1.190 | Ag+ | 0.75 mM, 40% inhibition | Secale cereale | |
2.4.1.190 | Cu2+ | 0.75 mM, 40% inhibition | Secale cereale | |
2.4.1.190 | luteolin | 0.1 mM, 50% inhibition | Secale cereale | |
2.4.1.190 | Pb2+ | 0.75 mM, 40% inhibition | Secale cereale | |
2.4.1.190 | UDP | - |
Secale cereale | |
2.4.1.191 | luteolin | 0.02 mM, 50% inhibition | Secale cereale | |
2.4.1.191 | UDP | strong | Secale cereale |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.189 | 0.008 | - |
luteolin | - |
Secale cereale | |
2.4.1.189 | 0.012 | - |
UDPglucuronic acid | - |
Secale cereale | |
2.4.1.190 | 0.012 | - |
luteolin 7-O-glucuronide | - |
Secale cereale | |
2.4.1.190 | 0.04 | - |
UDPglucuronic acid | - |
Secale cereale | |
2.4.1.191 | 0.009 | - |
Luteolin 7-O-diglucuronide | - |
Secale cereale | |
2.4.1.191 | 0.09 | - |
UDPglucuronic acid | - |
Secale cereale |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.189 | cytosol | probably | Secale cereale | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.189 | Ca2+ | 0.25 mM, 25% stimulation | Secale cereale | |
2.4.1.189 | Mg2+ | up to 1 mM, 20% stimulation | Secale cereale | |
2.4.1.191 | additional information | not affected by Ca2+ or Mg2+ up to 0.5 and 0.75 mM | Secale cereale |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.189 | 34500 | - |
gel filtration | Secale cereale |
2.4.1.190 | 37000 | - |
gel filtration | Secale cereale |
2.4.1.191 | 29000 | - |
gel filtration | Secale cereale |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.189 | UDPglucuronic acid + luteolin | Secale cereale | first enzyme in the biosynthesis of luteolin triglucuronide | UDP + luteolin 7-O-glucuronide | - |
ir | |
2.4.1.190 | UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide | Secale cereale | involved in the sequential biosynthesis of luteolin triglucuronide | UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
? | |
2.4.1.191 | UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | Secale cereale | involved in the sequential biosynthesis of luteolin triglucuronide | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.189 | Secale cereale | - |
rye | - |
2.4.1.190 | Secale cereale | - |
rye | - |
2.4.1.191 | Secale cereale | - |
rye | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.189 | ultrogel AcA 44, DEAE-cellulose, partial purification | Secale cereale |
2.4.1.190 | Ultrogel AcA 44, ultrafiltration, hydroxylapatite, partial purification | Secale cereale |
2.4.1.191 | Ultrogel AcA 44, Sephadex G-25, ultrafiltration, partial purification | Secale cereale |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.189 | leaf | - |
Secale cereale | - |
2.4.1.190 | leaf | - |
Secale cereale | - |
2.4.1.191 | leaf | - |
Secale cereale | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.189 | 1.16 | - |
- |
Secale cereale |
2.4.1.190 | 0.96 | - |
- |
Secale cereale |
2.4.1.191 | 0.14 | - |
- |
Secale cereale |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.189 | -20°C, 50% glycerol, 3 months, 30-50% loss of activity | Secale cereale |
2.4.1.190 | -20°C, 50% glycerol, 3 months, 30-50% loss of activity | Secale cereale |
2.4.1.191 | -20°C, 50% glycerol, 3 months, 30-50% loss of activity | Secale cereale |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.189 | UDP-alpha-D-glucose + luteolin | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
2.4.1.189 | UDPgalactose + luteolin | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-galactoside | - |
? | |
2.4.1.189 | UDPglucuronic acid + apigenin | 38% of activity compared to luteolin | Secale cereale | UDP + apigenin 7-O-glucuronide | - |
? | |
2.4.1.189 | UDPglucuronic acid + luteolin | - |
Secale cereale | UDP + luteolin 7-O-glucuronide | - |
ir | |
2.4.1.189 | UDPglucuronic acid + luteolin | first enzyme in the biosynthesis of luteolin triglucuronide | Secale cereale | UDP + luteolin 7-O-glucuronide | - |
ir | |
2.4.1.189 | UDPxylose + luteolin | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-xyloside | - |
? | |
2.4.1.190 | UDP-glucuronic acid + apigenin 7-O-beta-D-glucuronide | 88% of activity with luteolin 7-O-glucuronide | Secale cereale | UDP + apigenin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
? | |
2.4.1.190 | UDP-glucuronic acid + chrysoeriol 7-O-beta-D-glucuronide | 65% of activity with luteolin-7-O-glucuronide | Secale cereale | UDP + chrysoeriol 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
? | |
2.4.1.190 | UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide | - |
Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
ir | |
2.4.1.190 | UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide | involved in the sequential biosynthesis of luteolin triglucuronide | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
? | |
2.4.1.190 | UDPglucuronic acid + apigenin 7-O-glucoside | 51.5% of activity with luteolin 7-O-glucuronide | Secale cereale | UDP + apigenin-7-O-beta-D-glucosyl-1,4-beta-D-glucuronoside | - |
? | |
2.4.1.191 | UDP-galactose + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide] | 7.5% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-galactoside | - |
? | |
2.4.1.191 | UDP-glucose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucoside | - |
? | |
2.4.1.191 | UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir | |
2.4.1.191 | UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | involved in the sequential biosynthesis of luteolin triglucuronide | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir | |
2.4.1.191 | UDP-glucuronic acid + luteolin-7-O-glucuronide | - |
Secale cereale | UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide | - |
? | |
2.4.1.191 | UDP-xylose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-xyloside | - |
? | |
2.4.1.191 | UDPglucuronic acid + luteolin-7-O-glucuronide | 34% of activity with luteolin 7-O-diglucuronide | Secale cereale | UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.189 | 50 | - |
- |
Secale cereale |
2.4.1.190 | 52 | - |
- |
Secale cereale |
2.4.1.191 | 40 | - |
- |
Secale cereale |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.189 | 6.5 | - |
50% of maximal activity at pH 5.6 | Secale cereale |
2.4.1.190 | 6.5 | - |
- |
Secale cereale |
2.4.1.190 | 8.5 | - |
pH-optimum at pH 8.5 is absent in crude enzyme preparations | Secale cereale |
2.4.1.191 | 7 | - |
- |
Secale cereale |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.189 | 5.6 | 9 | 50% of maximal activity at pH 5.6 and pH 9.0 | Secale cereale |
2.4.1.190 | 6 | 9 | 50% of maximal activity at pH 6.0 and pH 9.0 | Secale cereale |
2.4.1.191 | 6 | 9 | 50% of maximal activity at pH 6 and pH 9 | Secale cereale |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.189 | 0.26 | - |
UDP | - |
Secale cereale | |
2.4.1.190 | 0.12 | - |
UDP | - |
Secale cereale |