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Literature summary extracted from

  • Kumita, K.; Murazumi, N.; Araki, Y.; Ito, E.
    Solubilization and properties of UDP-D-glucose:N-acetylglucosaminyl pyrophosphorylundecaprenol glucosyltransferase from Bacillus coagulans AHU 1366 membranes (1988), J. Biochem., 104, 985-988.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.188 (NH4)2SO4 stimulation Heyndrickxia coagulans
2.4.1.188 glycerol at 20% stimulates 3fold Heyndrickxia coagulans
2.4.1.188 KCl at 0.6 M stimulation Heyndrickxia coagulans
2.4.1.188 MgCl2 at 40 mM stimulation Heyndrickxia coagulans
2.4.1.188 NaCl stimulation Heyndrickxia coagulans
2.4.1.188 NH4Cl stimulation Heyndrickxia coagulans
2.4.1.188 Nonidet P-40 at 0.1% slight stimulatory Heyndrickxia coagulans

General Stability

EC Number General Stability Organism
2.4.1.188 stabilization of the solubilized enzyme by cations and glycerol Heyndrickxia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.188 0.021
-
 UDP-glucose
-
 Heyndrickxia coagulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.188 membrane
-
 Heyndrickxia coagulans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.188 UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol Heyndrickxia coagulans
-
 UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?
2.4.1.188 UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol Heyndrickxia coagulans AHU 1366
-
 UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.188 Heyndrickxia coagulans
-
 AHU 1366
-
2.4.1.188 Heyndrickxia coagulans AHU 1366
-
 AHU 1366
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.188 partial Heyndrickxia coagulans

Storage Stability

EC Number Storage Stability Organism
2.4.1.188 -18°C, several months without significant loss of activity Heyndrickxia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.188 UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 Heyndrickxia coagulans UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?
2.4.1.188 UDP-glucose + N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 Heyndrickxia coagulans AHU 1366 UDP + beta-D-glucosyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.188 25
-
 assay at Heyndrickxia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.188 6.6 8
-
 Heyndrickxia coagulans