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Literature summary extracted from

  • Durren, R.L.; McIntosh, C.A.
    Flavanone-7-O-glucosyltransferase activity from Petunia hybrida (1999), Phytochemistry, 52, 793-798.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.185 UDP competitive inhibition Petunia x hybrida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.185 0.008
-
naringenin peak III Petunia x hybrida
2.4.1.185 0.01
-
naringenin peak I Petunia x hybrida
2.4.1.185 0.027
-
naringenin peak II Petunia x hybrida
2.4.1.185 0.074
-
UDPglucose peak II Petunia x hybrida
2.4.1.185 0.269
-
UDPglucose peak I Petunia x hybrida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.185 additional information activity is not affected by CaCl2 or MgCl2 Petunia x hybrida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.185 54400
-
gel filtration Petunia x hybrida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone Petunia x hybrida peak I-III UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.185 Petunia x hybrida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.185 ammonium sulfate, Superose 12, Mono Q, 3 peaks with 7-O-glucosyltransferase activity Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.185 flower bud
-
Petunia x hybrida
-
2.4.1.185 leaf
-
Petunia x hybrida
-
2.4.1.185 sepal
-
Petunia x hybrida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone peak I-III Petunia x hybrida UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
?
2.4.1.185 UDP-alpha-D-glucose + apigenin peak I-III, 49%, 45% and 95% of activity with naringenin respectively Petunia x hybrida UDP + apigenin 7-O-beta-D-glucoside
-
?
2.4.1.185 UDP-alpha-D-glucose + kaempferol peak I-III, 50%, 46% and 70% of activity with naringenin respectively Petunia x hybrida UDP + kaempferol 7-O-beta-D-glucoside
-
?
2.4.1.185 UDP-alpha-D-glucose + luteolin peak I, 52% of activity with naringenin Petunia x hybrida UDP + luteolin 7-O-beta-D-glucoside
-
?
2.4.1.185 UDP-alpha-D-glucose + quercetin peak I, 42% of activity with naringenin Petunia x hybrida UDP + quercetin 7-O-beta-D-glucoside
-
?
2.4.1.185 UDP-glucose + hesperetin peak I, 70% of activity with naringenin Petunia x hybrida UDP + hesperetin 7-O-glucoside
-
?
2.4.1.185 UDP-glucose + naringenin
-
Petunia x hybrida UDP + naringenin 7-O-glucoside
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.185 7.5
-
50% of maximal activity at pH 6.5, 62% of maximal activity at pH 9.0 Petunia x hybrida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.185 0.00089
-
UDP peak I Petunia x hybrida
2.4.1.185 0.074
-
UDP peak II Petunia x hybrida