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Literature summary extracted from

  • Matern, H.; Matern, S.; Gerok, W.
    Isolation and characterization of rat liver microsomal UDP-glucuronosyltransferase activity toward chenodeoxycholic acid and testosterone as a single form of enzyme (1982), J. Biol. Chem., 257, 7422-7429.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 UDP-N-acetylglucosamine inhibition or chenodeoxycholic acid and testosterone glucuronidation Rattus norvegicus
2.4.1.17 UDPgalactose inhibition or chenodeoxycholic acid and testosterone glucuronidation Rattus norvegicus
2.4.1.17 UDPgalacturonic acid inhibition or chenodeoxycholic acid and testosterone glucuronidation Rattus norvegicus
2.4.1.17 UDPxylose inhibition or chenodeoxycholic acid and testosterone glucuronidation Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.17 316000 318000 gel filtration, polyacrylamide gradient slab gel electrophoresis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Rattus norvegicus
-
 Sprague-Dawley rat
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.17 glycoprotein no glycoprotein Rattus norvegicus
2.4.1.17 phospholipoprotein less than 3 mol phospholipid per mol of enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.17 UDP-alpha-D-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside sequential bireactant mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 0.168
-
 chenodeoxycholic acid, Sprague-Dawley rat treated with phenobarbital Rattus norvegicus
2.4.1.17 0.983
-
 testosterone, Sprague-Dawley rat treated with phenobarbital Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.17 -20°C, at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 chenodeoxycholic acid + UDP-glucuronate
-
 Rattus norvegicus UDP + ?
-
 ?
2.4.1.17 chenodeoxycholic acid + UDPgalacturonate
-
 Rattus norvegicus UDP + chenodeoxycholic acid O-galacturonide
-
 ?
2.4.1.17 testosterone + UDPglucuronate UDPglucuronate can be substituted by UDPgalacturonate yielding 83-88% of the activity Rattus norvegicus UDP + testosterone O-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? additional values for rat isozymes ranging from 51000 to 59000 Rattus norvegicus
2.4.1.17 ? all values determined by SDS-PAGE Rattus norvegicus