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Literature summary extracted from

  • Rathbone, E.B.; Hacking, A.J.; Cheetham, P.S.J.
    Trihalo-trideoxy galactosucrose compounds (1985), UK Patent Application, GB21, 45080 A.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.162 200
-
 sucrose strain NCIB 11871 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.162 extracellular
-
 Bacillus subtilis
-
-
2.4.1.162 extracellular
-
 Erwinia sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.162 fructose donor + fructose acceptor Bacillus subtilis
-
 D-aldose + D-aldosyl beta-D-fructoside the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides ?
2.4.1.162 fructose donor + fructose acceptor Erwinia sp.
-
 D-aldose + D-aldosyl beta-D-fructoside the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.162 Bacillus subtilis
-
 NCIB 11871, 11872, 11873
-
2.4.1.162 Erwinia sp.
-
 previously Aerobacter levanicum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.162
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.162 6-deoxy-D-glucose + sucrose
-
 Bacillus subtilis 6-deoxysucrose + D-glucose
-
 ?
2.4.1.162 alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
-
 Bacillus subtilis alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
-
 ?
2.4.1.162 alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
-
 Erwinia sp. alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
-
 ?
2.4.1.162 D-glucose 6-benzoate + sucrose
-
 Bacillus subtilis sucrose 6-benzoate + D-glucose
-
 ?
2.4.1.162 fructose donor + fructose acceptor
-
 Bacillus subtilis D-aldose + D-aldosyl beta-D-fructoside the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides ?
2.4.1.162 fructose donor + fructose acceptor
-
 Erwinia sp. D-aldose + D-aldosyl beta-D-fructoside the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides ?
2.4.1.162 fructose donor + fructose acceptor the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine Bacillus subtilis D-aldose + D-aldosyl beta-D-fructoside
-
 ?
2.4.1.162 fructose donor + fructose acceptor the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine Erwinia sp. D-aldose + D-aldosyl beta-D-fructoside
-
 ?
2.4.1.162 glucose 6-acetate + sucrose
-
 Bacillus subtilis sucrose 6-acetate + D-glucose
-
 ?
2.4.1.162 raffinose + galactose
-
 Bacillus subtilis beta-D-fructofuranosyl-2,1-D-galactopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
 ?
2.4.1.162 raffinose + xylose
-
 Bacillus subtilis beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
 ?
2.4.1.162 sucrose + 3-O-methyl alpha-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
-
 ?
2.4.1.162 sucrose + 3-O-methyl alpha-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
-
 ?
2.4.1.162 sucrose + 3-O-methyl-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
-
 ?
2.4.1.162 sucrose + 3-O-methyl-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
-
 ?
2.4.1.162 sucrose + 4-chloro-4-deoxy-D-galactose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
-
 ?
2.4.1.162 sucrose + 4-chloro-4-deoxy-D-galactose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
-
 ?
2.4.1.162 sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
-
 ?
2.4.1.162 sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
-
 ?
2.4.1.162 sucrose + 6-chloro-6-deoxy-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-chloro-6-deoxy-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-deoxy-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-deoxy-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-O-benzyl-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-O-benzyl-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-O-methyl-D-galactose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
-
 ?
2.4.1.162 sucrose + 6-O-methyl-D-galactose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
-
 ?
2.4.1.162 sucrose + 6-O-methyl-D-glucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
-
 ?
2.4.1.162 sucrose + 6-O-methyl-D-glucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
-
 ?
2.4.1.162 sucrose + cellobiose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside
-
 ?
2.4.1.162 sucrose + cellobiose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside
-
 ?
2.4.1.162 sucrose + D-arabinose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside
-
 ?
2.4.1.162 sucrose + D-arabinose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside
-
 ?
2.4.1.162 sucrose + D-galactose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside
-
 ?
2.4.1.162 sucrose + D-galactose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside
-
 ?
2.4.1.162 sucrose + D-galactose
-
 Bacillus subtilis beta-D-fructofuranosyl-2,1-D-galactopyranoside + D-glucose
-
 ?
2.4.1.162 sucrose + D-galactose 6-acetate
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
-
 ?
2.4.1.162 sucrose + D-galactose 6-acetate
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
-
 ?
2.4.1.162 sucrose + D-gluconic acid
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid
-
 ?
2.4.1.162 sucrose + D-gluconic acid
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid
-
 ?
2.4.1.162 sucrose + D-glucose 6-acetate
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
-
 ?
2.4.1.162 sucrose + D-glucose 6-acetate
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
-
 ?
2.4.1.162 sucrose + D-glucose 6-phosphate
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
-
 ?
2.4.1.162 sucrose + D-glucose 6-phosphate
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
-
 ?
2.4.1.162 sucrose + D-glycero-D-galactoheptose
-
 Bacillus subtilis D-glucose + ?
-
 ?
2.4.1.162 sucrose + D-glycero-D-galactoheptose
-
 Erwinia sp. D-glucose + ?
-
 ?
2.4.1.162 sucrose + D-mannose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside
-
 ?
2.4.1.162 sucrose + D-mannose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside
-
 ?
2.4.1.162 sucrose + D-ribose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside
-
 ?
2.4.1.162 sucrose + D-ribose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside
-
 ?
2.4.1.162 sucrose + D-xylose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside
-
 ?
2.4.1.162 sucrose + D-xylose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside
-
 ?
2.4.1.162 sucrose + ethanol
-
 Bacillus subtilis D-glucose + 2-O-ethyl-beta-D-fructofuranose
-
 ?
2.4.1.162 sucrose + ethanol
-
 Erwinia sp. D-glucose + 2-O-ethyl-beta-D-fructofuranose
-
 ?
2.4.1.162 sucrose + glycerol
-
 Bacillus subtilis D-glucose + ?
-
 ?
2.4.1.162 sucrose + glycerol
-
 Erwinia sp. D-glucose + ?
-
 ?
2.4.1.162 sucrose + isomaltose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside
-
 ?
2.4.1.162 sucrose + isomaltose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside
-
 ?
2.4.1.162 sucrose + L-arabinose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
-
 ?
2.4.1.162 sucrose + L-arabinose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
-
 ?
2.4.1.162 sucrose + L-fucose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside
-
 ?
2.4.1.162 sucrose + L-fucose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside
-
 ?
2.4.1.162 sucrose + L-rhamnose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside
-
 ?
2.4.1.162 sucrose + L-rhamnose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside
-
 ?
2.4.1.162 sucrose + lactose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside
-
 ?
2.4.1.162 sucrose + lactose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside
-
 ?
2.4.1.162 sucrose + lyxose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside
-
 ?
2.4.1.162 sucrose + lyxose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside
-
 ?
2.4.1.162 sucrose + maltohexaose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside
-
 ?
2.4.1.162 sucrose + maltohexaose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside
-
 ?
2.4.1.162 sucrose + maltopentaose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside
-
 ?
2.4.1.162 sucrose + maltopentaose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside
-
 ?
2.4.1.162 sucrose + maltose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside
-
 ?
2.4.1.162 sucrose + maltose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside
-
 ?
2.4.1.162 sucrose + maltotriose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside
-
 ?
2.4.1.162 sucrose + maltotriose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside
-
 ?
2.4.1.162 sucrose + melibiose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside
-
 ?
2.4.1.162 sucrose + melibiose
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside
-
 ?
2.4.1.162 sucrose + sorbose
-
 Bacillus subtilis D-glucose + ?
-
 ?
2.4.1.162 sucrose + sorbose
-
 Erwinia sp. D-glucose + ?
-
 ?
2.4.1.162 sucrose + xylitol
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol
-
 ?
2.4.1.162 sucrose + xylitol
-
 Erwinia sp. D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol
-
 ?
2.4.1.162 sucrose + xylose
-
 Bacillus subtilis beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.162 30
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.162 45
-
 stable at least 20 min Bacillus subtilis
2.4.1.162 45
-
 stable at least 20 min Erwinia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.162 5.4 6
-
 Bacillus subtilis