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Literature summary extracted from

  • Kang, M.S.; Elango, N.; Mattia, E.; Au-Young, J.; Robbins, P.W.; Cabib, E.
    Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product (1984), J. Biol. Chem., 259, 14966-14972.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 GlcNAc
-
 Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.4.1.16 upon lyophilization, about 70% of the activity is recovered Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 0.7
-
 UDP-GlcNAc
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.16 570000
-
 gel filtration, calculation from sedimentation coefficient Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.16
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.4.1.16 -80°C, quite stable for several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n the enzyme itself is capable both of initiating chitin chains without a primer and of determining their length Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 at low concentrations of UDP-GlcNAc, no insoluble chitin is formed. Instead N-acetylglucosamine is incorporated into water-soluble products ?

Subunits

EC Number Subunits Comment Organism
2.4.1.16 More in SDS-PAGE, the purified enzyme shows a major band of 63000 Da and a weaker band at 74000 Da Saccharomyces cerevisiae