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Literature summary extracted from

  • Orlean, P.
    Two chitin synthases in Saccharomyces cerevisiae (1987), J. Biol. Chem., 262, 5732-5739.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 Digitonin chitin synthase 2 is maximally stimulated at the sharply defined digitonin to protein ratio of 0.042. Chitin synthase I is maximally active at a digitonin to protein ratio of 0.3-0.75 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 Co2+ strongly inhibits chitin synthase 1 Saccharomyces cerevisiae
2.4.1.16 EDTA inhibition of chitin synthase 1 and 2 Saccharomyces cerevisiae
2.4.1.16 Mn2+ 2.5 mM, 50% inhibition of chitin synthase 1 and 2 Saccharomyces cerevisiae
2.4.1.16 Polyoxin D competitive Saccharomyces cerevisiae
2.4.1.16 Zn2+ 2.5 mM, complete inhibition of both chitin synthase 1 and 2 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 0.5
-
 UDP-GlcNAc chitin synthase 1 Saccharomyces cerevisiae
2.4.1.16 0.6 0.8 UDP-GlcNAc chitin synthase 2 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Mg2+ the best divalent cation stimulator of chitin synthase 1 and 2 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n Saccharomyces cerevisiae chitin synthetase 2 is responsible for chitin synthesis in vivo, chitin synthase 1 is not essential UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Saccharomyces cerevisiae
-
 chitin synthetase 1 and 2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.16 proteolytic modification chitin synthetase I exists as zymogen and is only measurable after trypsin treatment Saccharomyces cerevisiae
2.4.1.16 proteolytic modification chitin synthetase 2 is not activated by proteolysis Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.16 additional information chitin synthase II shows highest specific activities in extracts from logarithmically growing cultures. Chitin synthetase I, whether from growing cultures or stationary phase cultures, is only measurable after trypsin treatment, and levels of zymogen do not change Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n chitin synthetase 2 is responsible for chitin synthesis in vivo, chitin synthase 1 is not essential Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.16 25
-
 chitin synthase 1 Saccharomyces cerevisiae
2.4.1.16 40
-
 chitin synthase 2 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.16 20 40 20°C: about 80% of maximal activity, 40°C: about 55% of maximal activity Saccharomyces cerevisiae
2.4.1.16 25 50 25°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, chitin synthase 1 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.16 7
-
 chitin synthase 1 Saccharomyces cerevisiae
2.4.1.16 8
-
 chitin synthase 2 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.16 0.001
-
 Polyoxin D
-
 Saccharomyces cerevisiae