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Literature summary extracted from

  • Pan, Y.T.; Carroll, J.D.; Elbein, A.D.
    Trehalose-phosphate synthase of Mycobacterium tuberculosis. Cloning, expression and properties of the recombinant enzyme (2002), Eur. J. Biochem., 269, 6091-6100.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 heparin
-
 Mycobacterium tuberculosis
2.4.1.347 Mn2+ at 10 mM activity increases 2fold Mycobacterium tuberculosis

Application

EC Number Application Comment Organism
2.4.1.15 medicine the enzyme should be a valuable target for chemotherapeutic intervention in tuberculosis Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15
-
 Mycobacterium tuberculosis
2.4.1.347
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 4
-
 glucose-6-phosphate with GDPglucose as glucosyl donor Mycobacterium tuberculosis
2.4.1.15 7
-
 glucose-6-phosphate with UDPglucose as glucosyl donor Mycobacterium tuberculosis
2.4.1.15 16
-
 GDPglucose
-
 Mycobacterium tuberculosis
2.4.1.15 18
-
 UDPglucose
-
 Mycobacterium tuberculosis
2.4.1.347 4 7 glucose-6-phosphate
-
 Mycobacterium tuberculosis
2.4.1.347 16
-
 GDP-glucose
-
 Mycobacterium tuberculosis
2.4.1.347 18
-
 UDP-glucose
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 Mn2+ 10 mM, 2fold increase of activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.15 58000
-
 4 * 58000, SDS-PAGE Mycobacterium tuberculosis
2.4.1.15 220000
-
 gel filtration Mycobacterium tuberculosis
2.4.1.347 58000
-
 4 * 58000, SDS-PAGE, recombinant enzyme Mycobacterium tuberculosis
2.4.1.347 220000
-
 gel filtration, recombinant enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDPglucose + glucose 6-phosphate Mycobacterium tuberculosis
-
 trehalose 6-phosphate + UDP
-
 ?
2.4.1.347 GDP-glucose + glucose 6-phosphate Mycobacterium tuberculosis responsible for synthesis of trehalose phosphate GDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Mycobacterium tuberculosis
-
-
-
2.4.1.347 Mycobacterium tuberculosis
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.347 recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 ADP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 CDP-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis trehalose 6-phosphate + CDP
-
 ?
2.4.1.15 GDP-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis trehalose 6-phosphate + GDP
-
 ?
2.4.1.15 TDP-glucose + glucose 6-phosphate
-
 Mycobacterium tuberculosis trehalose 6-phosphate + TDP
-
 ?
2.4.1.15 UDPglucose + glucose 6-phosphate
-
 Mycobacterium tuberculosis trehalose 6-phosphate + UDP
-
 ?
2.4.1.15 UDPglucose + glucose 6-phosphate absolute specificity for glucose 6-phosphate Mycobacterium tuberculosis trehalose 6-phosphate + UDP
-
 ?
2.4.1.347 ADP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 CDP-glucose + glucose 6-phosphate
-
 Mycobacterium tuberculosis CDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 GDP-glucose + glucose 6-phosphate
-
 Mycobacterium tuberculosis GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 GDP-glucose + glucose 6-phosphate responsible for synthesis of trehalose phosphate Mycobacterium tuberculosis GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 TDP-glucose + glucose 6-phosphate
-
 Mycobacterium tuberculosis TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.15 tetramer 4 * 58000, SDS-PAGE Mycobacterium tuberculosis
2.4.1.347 tetramer 4 * 58000, SDS-PAGE, recombinant enzyme Mycobacterium tuberculosis