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Literature summary extracted from
- Partial purification and properties of UDP-glucose: vanillate 1-O-glucosyl transferase from oak leaves (1983), Phytochemistry, 22, 2179-2182.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.136 | 2-mercaptoethanol in extraction buffers stabilizes | Quercus rubra |
| 2.4.1.136 | diluted enzyme is unstable | Quercus rubra |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.136 | dithiothreitol | 4 mM, slight | Quercus rubra |  |
| 2.4.1.136 | UDP | strong | Quercus rubra | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.136 | 0.57 | - |
vanillate | - |
Quercus rubra | |
| 2.4.1.136 | 0.72 | - |
veratrate | - |
Quercus rubra | |
| 2.4.1.136 | 1.11 | - |
gallate | - |
Quercus rubra | |
| 2.4.1.136 | 3.45 | - |
p-hydroxybenzoate | - |
Quercus rubra | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.136 | 68000 | - |
gel filtration | Quercus rubra |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.136 | UDPglucose + gallate | Quercus rubra | physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins | UDP + 1-O-galloyl-beta-D-glucose | i.e. beta-glucogallin | r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.136 | Quercus rubra | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 2.4.1.136 | O2-sensitive, mercaptoethanol stabilizes | Quercus rubra |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.136 | - |
Quercus rubra |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.136 | leaf | - |
Quercus rubra | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.136 | 0.0117 | - |
reaction with gallate | Quercus rubra |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.136 | 0-4°C, 1-2 weeks | Quercus rubra |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.136 | UDP-alpha-D-glucose + 4-hydroxybenzoate | 48% of activity compared to vanillate | Quercus rubra | UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucopyranose | - |
? | |
| 2.4.1.136 | UDP-alpha-D-glucose + cinnamate | 9% of activity compared to vanillate | Quercus rubra | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDP-glucose + p-coumarate | 32% of activity compared to vanillate | Quercus rubra | UDP + 1-O-p-coumaroyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + 3,4,5-trimethoxybenzoate | 7% of activity compared to vanillate | Quercus rubra | UDP + 1-O-3,4,5-trimethoxybenzoyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + anisate | 38% of activity compared to vanillate | Quercus rubra | UDP + 1-O-anisoyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + benzoate | 16% of activity compared to vanillate | Quercus rubra | UDP + benzoyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + ferulate | 35% of activity compared to vanillate | Quercus rubra | UDP + 1-O-feruloyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + gallate | 52% of activity compared to vanillate | Quercus rubra | UDP + 1-O-galloyl-beta-D-glucose | i.e. beta-glucogallin | r | |
| 2.4.1.136 | UDPglucose + gallate | physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins | Quercus rubra | UDP + 1-O-galloyl-beta-D-glucose | i.e. beta-glucogallin | r | |
| 2.4.1.136 | UDPglucose + m-coumarate | 14% of activity compared to vanillate | Quercus rubra | UDP + 1-O-m-coumaroyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + protocatechuate | 22% of activity compared to vanillate | Quercus rubra | UDP + protocatechuyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + sinapate | 13% of activity compared to vanillate | Quercus rubra | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + vanillate | best substrate | Quercus rubra | UDP + vanilloyl-beta-D-glucose | - |
? | |
| 2.4.1.136 | UDPglucose + veratrate | 71% of activity compared to vanillate | Quercus rubra | UDP + 1-O-veratroyl-beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.136 | 42 | - |
- |
Quercus rubra |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.136 | 6.5 | 7 | - |
Quercus rubra |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.136 | 4.7 | 9 | 50% of maximal activity at pH 4.7 and 9.0 | Quercus rubra |
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