Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Echt, C.S.; Chourey, P.S.
    A comparison of two sucrose synthetase isozymes from normal and shrunken-1 maize (1985), Plant Physiol., 79, 530-536.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.1.13 repeated freeze-thawing leads to slight decrease of activity, even in the presence of PMSF Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 52 290 sucrose
-
 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 additional information
-
 amino acid composition Zea mays
2.4.1.13 87000
-
 x * 87000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Zea mays
-
 maize, isozymes SS1, SS2, sh1/sh1- (Black Mexican Sweet) or sh1bz1-m4-genotype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 2 isozymes: SS1 and SS2 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 cell suspension culture
-
 Zea mays
-
2.4.1.13 endosperm
-
 Zea mays
-
2.4.1.13 seedling
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.13 6
-
 isozyme SS2 Zea mays
2.4.1.13 10
-
 isozyme SS1 Zea mays

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 -80°C, 20% glycerol, 0.1-1.0 mM DTT, stable Zea mays
2.4.1.13 4°C, prolonged storage leads to slight decrease of activity, even in the presence of PMSF Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 UDP + sucrose also substrate: ADP Zea mays UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose substrate: TDP Zea mays UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose with SS2-isozyme TDP is 57%, with SS1-isozyme 91% as effective as UDP Zea mays UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose ADP is less effective Zea mays UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDPglucose + D-fructose
-
 Zea mays UDP + sucrose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 oligomer x * 87000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 additional information
-
 pI: 5.8, isozyme SS1 Zea mays