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Literature summary extracted from

  • Su, J.C.; Preiss, J.
    Purification and properties of sucrose synthase from maize (1978), Plant Physiol., 61, 389-393.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.1.13 repeated freeze-thawing, stable to if foaming is avoided Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 Mn2+ 3 mM Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Mg2+ activation Zea mays
2.4.1.13 Mg2+ sucrose synthesis, 0.1-10.0 mM Zea mays
2.4.1.13 Mn2+ activation Zea mays
2.4.1.13 Mn2+ inhibits at 3 mM Zea mays
2.4.1.13 Mn2+ 0.1 mM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 additional information
-
 amino acid composition Zea mays
2.4.1.13 88000
-
 4 * 88000, SDS-PAGE, depending on the ionic species and ionic strength of the solution, the enzyme can aquire catalytically active, tetrameric, octameric and other higher aggregated forms of which the tetramer is the predominant form Zea mays
2.4.1.13 360000
-
 analytical ultracentrifugation in the presence of Mg2+ Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 seed
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.13 3.85
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 -20°C, about 75% of activity retained after 4 months Zea mays
2.4.1.13 4°C, storage leads to gradual precipitation of denatured protein Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 UDP + sucrose
-
 Zea mays UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDPglucose + D-fructose
-
 Zea mays UDP + sucrose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 tetramer 4 * 88000, SDS-PAGE, depending on the ionic species and ionic strength of the solution, the enzyme can aquire catalytically active, tetrameric, octameric and other higher aggregated forms of which the tetramer is the predominant form Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 60
-
 and above, 5 min, rapid loss of activity Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 6
-
 sucrose cleavage Zea mays

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.13 5
-
 below, complete inactivation Zea mays
2.4.1.13 5.5 8 stable Zea mays
2.4.1.13 8
-
 above, gradual loss of activity Zea mays