EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.10 | constitutive overexpression in Escherichia coli DH5alpha under control of Rahnella aquatilis levansucrase promotor | Zymomonas mobilis |
2.4.1.10 | expression in Escherichia coli as His-tagged protein | Zymomonas mobilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 46000 | - |
x * 46000, SDS-PAGE | Zymomonas mobilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.10 | Zymomonas mobilis | Q60114 | - |
- |
2.4.1.10 | Zymomonas mobilis ZM1 | Q60114 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.10 | recombinant as soluble His-tagged protein, small scale | Zymomonas mobilis |
2.4.1.10 | recombinant from inclusion bodies in E. coli cytoplasm, direct refolding and solubilization with Triton X-100, large scale in fed-batch culture of recombinant E. coli | Zymomonas mobilis |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.4.1.10 | refolding after treatment of inclusion bodies with high conecntrations of urea and guanidine hydrochloride is not successful, formation of large amounts of insoluble aggregates | Zymomonas mobilis |
2.4.1.10 | refolding and solubilization of recombinant enzyme from inclusion bodies in E. coli by Triton X-100 4% v/v, maximal at pH 6.4-6.8 with 0.3-0.5 M glycine, which enhanced the refolding efficiency | Zymomonas mobilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.10 | sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
2.4.1.10 | sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis ZM1 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.10 | ? | x * 46000, SDS-PAGE | Zymomonas mobilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 37 | - |
assay at | Zymomonas mobilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 6 | - |
assay at | Zymomonas mobilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 5 | 8.4 | recombinant enzyme | Zymomonas mobilis |