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Literature summary extracted from

  • Song, K.B.; Seo, J.W.; Kim, M.G.; Rhee, S.K.
    Levansucrase of Rahnella aquatilis ATCC33071: gene cloning, expression, and levan formation (1998), Ann. N. Y. Acad. Sci., 864, 506-511.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.10 synthesis enzyme is an important biocatalyst for production of fructose homopolymers, potential usage in bioindustrial fields Rahnella aquatilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression of His-tagged enzyme in Escherichia coli DH5alpha Rahnella aquatilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 additional information
-
 amino acid sequence alignment Zymomonas mobilis
2.4.1.10 additional information
-
 amino acid sequence alignment Rahnella aquatilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Rahnella aquatilis O54435 strain ATCC33071, gene lsrA
-
2.4.1.10 Rahnella aquatilis ATCC 33071 O54435 strain ATCC33071, gene lsrA
-
2.4.1.10 Zymomonas mobilis
-
 gene levU
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 recombinant from E. coli as His-tagged protein Rahnella aquatilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.10 sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats Zymomonas mobilis
a
2.4.1.10 sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats Rahnella aquatilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n transfucosylation from sucrose to various acceptors, except sugar alcohols Rahnella aquatilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature Rahnella aquatilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n transfucosylation from sucrose to various acceptors, except sugar alcohols Rahnella aquatilis ATCC 33071 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature Rahnella aquatilis ATCC 33071 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 additional information
-
-
 Zymomonas mobilis
2.4.1.10 additional information
-
 the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature Rahnella aquatilis
2.4.1.10
-
-
-
 Zymomonas mobilis
2.4.1.10 20
-
 levan production Rahnella aquatilis
2.4.1.10 40
-
 levan formation Rahnella aquatilis
2.4.1.10 50
-
-
 Zymomonas mobilis
2.4.1.10 50
-
 maximal reaction velocity Rahnella aquatilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 4 6
-
 Zymomonas mobilis
2.4.1.10 4 6 recombinant enzyme Rahnella aquatilis