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Literature summary extracted from

  • Hettwer, U.; Gross, M.; Rudolph, K.
    Purification and characterization of an extracellular levansucrase from Pseudomonas syringae pv. phaseolicola (1995), J. Bacteriol., 177, 2834-2839.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.10 elugent slight activation at 1% Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 Tween 20 slight activation at 1% Pseudomonas savastanoi pv. phaseolicola

General Stability

EC Number General Stability Organism
2.4.1.10 1% deoxycholate reduces the activity by 4%, 10% SDS decreases the activity by 27% Pseudomonas savastanoi pv. phaseolicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 D-glucose
-
 Bacillus subtilis
2.4.1.10 D-glucose complete inhibition at 30 mM Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 D-glucose complete inhibition at 30 mM Zymomonas mobilis
2.4.1.10 mannose no inhibition Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 additional information arabinose is not inhibitory Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 additional information
-
 Zymomonas mobilis
2.4.1.10 NaCl 30% of the protein is precipitated at 2 M NaCl Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 proteinase K proteinase K has no effect Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 Urea 6 M urea decreased the activity by 40% Pseudomonas savastanoi pv. phaseolicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 160
-
 sucrose
-
 Pseudomonas savastanoi pv. phaseolicola

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 extracellular
-
 Bacillus subtilis
-
-
2.4.1.10 extracellular
-
 Zymomonas mobilis
-
-
2.4.1.10 extracellular
-
 Pseudomonas savastanoi pv. phaseolicola
-
-
2.4.1.10 extracellular
-
 Streptococcus mutans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 additional information
-
 amino acid N-terminal sequence alignment Bacillus subtilis
2.4.1.10 additional information
-
 amino acid N-terminal sequence alignment Zymomonas mobilis
2.4.1.10 additional information
-
 amino acid N-terminal sequence alignment Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 additional information
-
 amino acid N-terminal sequence alignment Streptococcus mutans
2.4.1.10 additional information
-
 amino acid N-terminal sequence alignment Erwinia amylovora
2.4.1.10 45000
-
 x * 45000, SDS-PAGE Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 68000
-
 gel filtration Pseudomonas savastanoi pv. phaseolicola

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus subtilis
-
-
-
2.4.1.10 Erwinia amylovora
-
-
-
2.4.1.10 Pseudomonas savastanoi pv. phaseolicola
-
-
-
2.4.1.10 Streptococcus mutans
-
-
-
2.4.1.10 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10
-
 Pseudomonas savastanoi pv. phaseolicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 298
-
 purified enzyme Pseudomonas savastanoi pv. phaseolicola

Storage Stability

EC Number Storage Stability Organism
2.4.1.10 4°C, no loss of activity, 6 months Pseudomonas savastanoi pv. phaseolicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Bacillus subtilis glucose + (2,6-beta-D-fructosyl)n+1
-
 r
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
 r
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Streptococcus mutans glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Pseudomonas savastanoi pv. phaseolicola glucose + (2,6-beta-D-fructosyl)n+1 molecular weight of levan product is dependent on temperature, salinity, and sucrose concentration r

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ?
-
 Bacillus subtilis
2.4.1.10 ? x * 45000, SDS-PAGE Pseudomonas savastanoi pv. phaseolicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 18
-
 levan synthesis Pseudomonas savastanoi pv. phaseolicola
2.4.1.10 60
-
 sucrose hydrolysis Pseudomonas savastanoi pv. phaseolicola

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.10 18 60
-
 Pseudomonas savastanoi pv. phaseolicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6
-
-
 Bacillus subtilis
2.4.1.10 6
-
-
 Pseudomonas savastanoi pv. phaseolicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 4.8 6.8 about half-maximal activity at pH 4.8 and pH 6.8 Pseudomonas savastanoi pv. phaseolicola