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Literature summary extracted from

  • Lyness, E.W.; Doelle, H.W.
    Levansucrase from Zymomonas mobilis (1983), Biotechnol. Lett., 5, 345-350.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.10 aspartic acid 5 mM, activates partially purified enzyme Zymomonas mobilis
2.4.1.10 fructose 1,6-biphosphate 5 mM, activates partially purified enzyme Zymomonas mobilis
2.4.1.10 glucoseamine-HCl 5 mM, activates partially purified enzyme Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 6-phosphogluconate reaction intermediate Zymomonas mobilis
2.4.1.10 D-glucose complete inhibition at 30 mM Zymomonas mobilis
2.4.1.10 ethanol complete inhibition at 1.6 M Zymomonas mobilis
2.4.1.10 Hg2+ no inhibition by HgCl2, 5 mM Zymomonas mobilis
2.4.1.10 mannose
-
 Zymomonas mobilis
2.4.1.10 additional information not affected by 2-mercaptoethanol; not affected by cysteine; not affected by several hexoses, overview Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 8
-
 sucrose
-
 Zymomonas mobilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.10 additional information not affected by 5 mM of Ba2+, Zn2+, Hg2+, Ni2+, Mn2+, Cu2+, Co2+, Ca2+, Na2MoO4 Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 120000
-
 gel filtration Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 additional information Zymomonas mobilis 6-beta-D-fructosyl)n is levan ?
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Zymomonas mobilis
-
 glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 partial Zymomonas mobilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 additional information
-
-
 Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 additional information 6-beta-D-fructosyl)n is levan Zymomonas mobilis ?
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1 enzyme plays an important role in the production of ethanol from sugar cane and other sucrose sources ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
 assay at Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6.5
-
 hydrolysis of sucrose to glucose and fructose Zymomonas mobilis