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Literature summary extracted from

  • Mu, H.H.; Yu, Y.; Wasserman, B.P.; Carman, G.M.
    Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase (2001), Arch. Biochem. Biophys., 388, 155-164.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose 2.5 mM, 80% inhibition of stromal phosphorylase Zea mays
2.4.1.1 diethyldicarbonate 0.84 mM, 50% inhibition Zea mays
2.4.1.1 Hg2+ 1 mM, 77% inhibition of stromal phosphorylase Zea mays
2.4.1.1 p-chloromercuriphenylsulfonic acid 1 mM, 88% inhibition of stromal phosphorylase Zea mays
2.4.1.1 Phenylglyoxal 12 mM, 50% inhibition Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.017
-
 amylopectin stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.028
-
 amylopectin stromal phosphorylase, phosphorolysis Zea mays
2.4.1.1 0.08
-
 maltoheptaose stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.1
-
 maltoheptaose stromal phosphorylase, phosphorolysis Zea mays
2.4.1.1 0.16
-
 maltohexaose stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.21
-
 maltohexaose stromal phosphorylase, phosphorolysis Zea mays
2.4.1.1 0.23
-
 maltopentaose stromal phosphorylase, phosphorolysis Zea mays
2.4.1.1 0.25
-
 glycogen stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.27
-
 maltopentaose stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.57
-
 maltotetraose stromal phosphorylase, synthesis Zea mays
2.4.1.1 0.94
-
 glycogen stromal phosphorylase, phosphorolysis Zea mays
2.4.1.1 1.51
-
 glucose 1-phosphate stromal phosphorylase Zea mays
2.4.1.1 2.26
-
 phosphate stromal phosphorylase Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 amyloplast stroma Zea mays 9501
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 112000
-
 2 * 112000, stromal phosphorylase, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 stromal phosphorylase, ammonium sulfate, Sephacryl 300 Hr, starch adsorption, Q-Sepharose, Mono Q Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 kernel
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 67.5
-
 stromal phosphorylase Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + glucose 1-phosphate
-
 Zea mays amylopectin + phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate
-
 Zea mays glycogen + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 112000, stromal phosphorylase, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 40
-
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 50
-
 stromal phosphorylase, 10% losss of activity after 15 min Zea mays
2.4.1.1 60
-
 stromal phosphorylase, 93% loss of activity after 15 min Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.5 7 phosphorolysis Zea mays
2.4.1.1 5.5 6.5 synthesis Zea mays