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Literature summary extracted from

  • Boeck, B.; Schinzel, R.
    Purification and characterization of an alpha-glucan phosphorylase from the thermophilic bacterium Thermus thermophilus (1996), Eur. J. Biochem., 239, 150-155.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 alpha-D-glucose-1-methylenephosphonate competitive vs. glucose 1-phosphate Escherichia coli
2.4.1.1 alpha-D-glucose-1-methylenephosphonate competitive vs. glucose 1-phosphate Thermus thermophilus
2.4.1.1 arsenate
-
 Escherichia coli
2.4.1.1 arsenate
-
 Thermus thermophilus
2.4.1.1 gluconolactone
-
 Escherichia coli
2.4.1.1 gluconolactone
-
 Thermus thermophilus
2.4.1.1 [(alpha-D-glucopyranosyloxy)methyl]phosphonic acid competitive vs. glucose 1-phosphate Escherichia coli
2.4.1.1 [(alpha-D-glucopyranosyloxy)methyl]phosphonic acid competitive vs. glucose 1-phosphate Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.2
-
 maltoheptaose phosphorolysis Escherichia coli
2.4.1.1 0.5
-
 phosphate phosphorolysis Escherichia coli
2.4.1.1 1
-
 glucose 1-phosphate synthesis Escherichia coli
2.4.1.1 1.2
-
 glucose 1-phosphate synthesis Thermus thermophilus
2.4.1.1 1.7
-
 maltoheptaose phosphorolysis, at 30°C Thermus thermophilus
2.4.1.1 1.9
-
 phosphate phosphorolysis, at 30°C Thermus thermophilus
2.4.1.1 2.2
-
 phosphate phosphorolysis, at 70°C Thermus thermophilus
2.4.1.1 2.8
-
 maltotetraose phosphorolysis, at 30°C Thermus thermophilus
2.4.1.1 3.6
-
 maltotetraose synthesis Escherichia coli
2.4.1.1 3.6
-
 maltoheptaose phosphorolysis, at 70°C Thermus thermophilus
2.4.1.1 3.9
-
 maltotetraose phosphorolysis Escherichia coli
2.4.1.1 4.8
-
 maltotetraose phosphorolysis, at 70°C Thermus thermophilus
2.4.1.1 12.7
-
 maltotetraose synthesis Thermus thermophilus
2.4.1.1 13.4
-
 maltotriose synthesis Thermus thermophilus
2.4.1.1 24.5
-
 maltotriose synthesis Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 93000
-
 2 * 93000, most likely subunit composition, SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Escherichia coli
-
-
-
2.4.1.1 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, Q-Sepharose, Mono Q, gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate
-
 Thermus thermophilus (dextrin)n + phosphate
-
 r
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate
-
 Escherichia coli (dextrin)n + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate
-
 Thermus thermophilus glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate
-
 Escherichia coli glycogen + phosphate
-
 r
2.4.1.1 maltoheptaose + phosphate
-
 Escherichia coli maltohexaose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltohexaose + glucose 1-phosphate
-
 Thermus thermophilus maltoheptaose + phosphate
-
 r
2.4.1.1 maltotetraose + glucose 1-phosphate
-
 Thermus thermophilus maltopentaose + phosphate
-
 r
2.4.1.1 maltotetraose + phosphate
-
 Escherichia coli maltotriose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltotriose + glucose 1-phosphate
-
 Thermus thermophilus maltotetraose + phosphate
-
 r
2.4.1.1 maltotriose + phosphate
-
 Escherichia coli maltobiose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Thermus thermophilus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Escherichia coli [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 93000, most likely subunit composition, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 70
-
 50% activity at 90°C Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 70
-
 half-life: 10 h Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.5 6.5
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate
-
 Escherichia coli
2.4.1.1 pyridoxal 5'-phosphate 1.2 mol/subunit Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.1
-
 alpha-D-glucose-1-methylenephosphonate
-
 Thermus thermophilus
2.4.1.1 0.4
-
 alpha-D-glucose-1-methylenephosphonate
-
 Escherichia coli
2.4.1.1 0.5
-
 arsenate
-
 Escherichia coli
2.4.1.1 0.9
-
 gluconolactone
-
 Escherichia coli
2.4.1.1 3.3
-
 gluconolactone
-
 Thermus thermophilus
2.4.1.1 4.1
-
 arsenate
-
 Thermus thermophilus