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Literature summary extracted from

  • Medicus, R.; Mendicino, J.
    Role of enzyme interactions in the regulation of glycolysis and gluconeogenesis. Purification and properties of the phospho- and dephospho-forms of glycogen phosphorylase from swine kidney (1973), Eur. J. Biochem., 40, 63-75.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Sus scrofa
2.4.1.1 5'-AMP kidney dephosphorylase is not activated by AMP Sus scrofa
2.4.1.1 5'-AMP required for dephospho-phosphorylase, kinetics Sus scrofa
2.4.1.1 phosphate activation Sus scrofa
2.4.1.1 Phosphorylase kinase activation Sus scrofa
2.4.1.1 protamine stimulation, dephospho-phosphorylase, kinetics Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 D-glucose 1 mM, significant inhibition of kidney dephosphophosphorylase Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 1.4
-
 glycogen kidney phosphophosphorylase Sus scrofa
2.4.1.1 2.3
-
 phosphate kidney phosphophosphorylase, in the presence of 0.8 mg glycogen Sus scrofa
2.4.1.1 2.6
-
 phosphate kidney phosphophosphorylase, in the presence of 0.4 mg glycogen Sus scrofa
2.4.1.1 2.8
-
 phosphate kidney phosphophosphorylase, in the presence of 0.2 mg glycogen Sus scrofa
2.4.1.1 2.9
-
 phosphate kidney phosphophosphorylase, in the presence of 0.1 mg glycogen Sus scrofa
2.4.1.1 20
-
 phosphate glycogen, dephospho-phosphorylase Sus scrofa
2.4.1.1 30
-
 phosphate kidney dephosphophosphorylase, in the presence of 1.6 mg glycogen Sus scrofa
2.4.1.1 70
-
 phosphate kidney dephosphophosphorylase, in the presence of 0.8 mg glycogen Sus scrofa
2.4.1.1 100
-
 phosphate dephospho-phosphorylase Sus scrofa
2.4.1.1 100
-
 phosphate kidney dephosphophosphorylase, in the presence of 0.2 mg glycogen Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Sus scrofa glycogen metabolism [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 side-chain modification activation by phosphorylation Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 alumina C-gamma gel, calcium phosphate gel, protamine sulfate, acetone, ethanol, ammonium sulfate, DEAE-cellulose, hydroxyapatite Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 30
-
 kidney dephospho-phosphorylase Sus scrofa
2.4.1.1 70
-
 kidney phospho-phosphorylase Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 -20°C, partially purified, lyophilized, at least 2 months Sus scrofa
2.4.1.1 -20°C, purified, lyophilized, 30% loss of activity within 3 months Sus scrofa
2.4.1.1 3°C, 2 weeks, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate
-
 Sus scrofa glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate glycogen metabolism Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer SDS-PAGE Sus scrofa