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Literature summary extracted from

  • Schliselfeld, L.H.
    Comparative studies of phosphorylase isozymes from the rabbit (1973), Ann. N. Y. Acad. Sci., 210, 181-191.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Sus scrofa
2.4.1.1 5'-AMP activation Oryctolagus cuniculus
2.4.1.1 5'-AMP phosphorylase Ia, half-maximal activity at 0.25 mM, phosphorylase IIIa, half-maximal activity at 6 mM Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 Antibodies to skeletal muscle phosphorylase heart enzyme Oryctolagus cuniculus
2.4.1.1 ATP complete inhibition of heart phosphorylase IIIb Oryctolagus cuniculus
2.4.1.1 ATP
-
 Sus scrofa
2.4.1.1 glucose 6-phosphate complete inhibition of heart phosphorylase IIIb Oryctolagus cuniculus
2.4.1.1 glucose 6-phosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 6
-
 glucose 1-phosphate heart phosphorylase I and III Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Oryctolagus cuniculus
-
-
-
2.4.1.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 heart
-
 Oryctolagus cuniculus
-
2.4.1.1 liver
-
 Sus scrofa
-
2.4.1.1 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 2
-
 liver phosophorylase b, in the presence of 1 mM AMP Oryctolagus cuniculus
2.4.1.1 13
-
 liver phosphorylase b, in the presence of 700 mM Na2SO4 Oryctolagus cuniculus
2.4.1.1 50
-
 liver phosphorylase a Oryctolagus cuniculus
2.4.1.1 54
-
 heart phosphorylase IIIa Oryctolagus cuniculus
2.4.1.1 64
-
 heart phosphorylase Ia Oryctolagus cuniculus
2.4.1.1 70
-
 heart phosphorylase Ib, in the presence of 1 mM AMP Oryctolagus cuniculus
2.4.1.1 70
-
 liver phosophorylase a, in the presence of 1 mM AMP Oryctolagus cuniculus
2.4.1.1 80
-
 heart phosphorylase IIIa, in the presence of 1 mM AMP Oryctolagus cuniculus
2.4.1.1 82
-
 heart phosphorylase Ia, in the presence of 1 mM AMP Oryctolagus cuniculus
2.4.1.1 88
-
 heart phosphorylase IIIb, in the presence of 1 mM AMP Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 0°C, heart isozyme Ia, stable in the presence of 2-mercaptoethanol Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 glycogen + glucose 1-phosphate
-
 Sus scrofa glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate
-
 Oryctolagus cuniculus glycogen + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Sus scrofa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Oryctolagus cuniculus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1
-
-
 heart isozyme Ib or IIIb: t1/2: 2.4-2.9 min, heart isozyme Ia: stable in the presence of 2-mercaptoethanol Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.4 6.7 heart isozyme IIIb Oryctolagus cuniculus
2.4.1.1 6.5 6.9 heart isozyme Ib Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate requirement Sus scrofa
2.4.1.1 pyridoxal 5'-phosphate requirement Oryctolagus cuniculus
2.4.1.1 pyridoxal 5'-phosphate 2 mol per mol enzyme Oryctolagus cuniculus