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Literature summary extracted from

  • Shivaram, K.N.
    Purification and properties of potato phosphorylase isozymes (1976), Z. Naturforsch. C, 31, 424-432.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 2-mercaptoethanol slight activation Solanum tuberosum
2.4.1.1 cysteine activation Solanum tuberosum
2.4.1.1 cysteine slight Solanum tuberosum
2.4.1.1 glutathione slight stimulation Solanum tuberosum
2.4.1.1 additional information not activated by indoleacetic acid and gibberellic acid Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 Ag+ 1 mM, complete inhibition Solanum tuberosum
2.4.1.1 Fe2+ 1 mM, approx. 40% inhibition of synthesis Solanum tuberosum
2.4.1.1 Hg2+ 1 mM, complete inhibition Solanum tuberosum
2.4.1.1 additional information not inhibited by 2-mercaptoethanol; not inhibited by agarose; not inhibited by gibberellic acid, indolyl-3-acetic acid; not inhibited by Ni2+ Solanum tuberosum
2.4.1.1 p-chloromercuribenzoate
-
Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
additional information kinetic data Solanum tuberosum
2.4.1.1 0.5
-
starch fast isoenzyme, starch phosphorolysis Solanum tuberosum
2.4.1.1 0.8
-
starch slow isoenzyme, starch phosphorolysis Solanum tuberosum
2.4.1.1 1.1
-
amylose fast isoenzyme, amylose phosphorolysis Solanum tuberosum
2.4.1.1 1.3
-
glycogen fast isoenzyme, glycogen phosphorolysis Solanum tuberosum
2.4.1.1 3.1
-
amylose slow isoenzyme, amylose phosphorolysis Solanum tuberosum
2.4.1.1 6.5
-
glycogen slow isoenzyme, glycogen phosphorolysis Solanum tuberosum
2.4.1.1 6.7
-
starch slow isoenzyme, starch synthesis Solanum tuberosum
2.4.1.1 8
-
starch fast isoenzyme, starch synthesis Solanum tuberosum
2.4.1.1 14.3
-
amylose slow isoenzyme, amylose synthesis Solanum tuberosum
2.4.1.1 20
-
amylose fast isoenzyme, amylose synthesis Solanum tuberosum
2.4.1.1 22.2
-
glycogen slow isoenzyme, glycogen synthesis Solanum tuberosum
2.4.1.1 40
-
glycogen fast isoenzyme, glycogen synthesis Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 Mg2+ slight activation Solanum tuberosum
2.4.1.1 Mg2+ activation Solanum tuberosum
2.4.1.1 Mn2+ activation Solanum tuberosum
2.4.1.1 Mn2+ slight Solanum tuberosum
2.4.1.1 additional information not activated by Cu2+ Solanum tuberosum
2.4.1.1 additional information not activated by Zn2+ Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 40000
-
x * 40000, fast isozyme, SDS-PAGE Solanum tuberosum
2.4.1.1 104000
-
x * 104000, slow isozyme, SDS-PAGE Solanum tuberosum
2.4.1.1 165000
-
fast isozyme, gel filtration Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Solanum tuberosum
-
cv. Adelheid or Rosa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 2 isozymes: termed slow and fast, heat treatment, ammonium sulfate, DEAE-cellulose, preparative gel electrophoresis Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 1.61
-
fast isozyme Solanum tuberosum
2.4.1.1 2.67
-
slow isozyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylose + glucose 1-phosphate
-
Solanum tuberosum amylose + phosphate
-
?
2.4.1.1 glycogen + glucose 1-phosphate
-
Solanum tuberosum glycogen + phosphate
-
?
2.4.1.1 starch + glucose 1-phosphate best substrate Solanum tuberosum starch + phosphate
-
r
2.4.1.1 starch + glucose 1-phosphate soluble starch Solanum tuberosum starch + phosphate
-
r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
Solanum tuberosum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 40000, fast isozyme, SDS-PAGE Solanum tuberosum
2.4.1.1 ? x * 104000, slow isozyme, SDS-PAGE Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 30 35 slow isozyme Solanum tuberosum
2.4.1.1 35
-
fast isozyme Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 55
-
unprimed phosphorylase II, 80% loss of activity after 10 min, albumin protects Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 additional information
-
pI: 5.0, slow isozyme Solanum tuberosum
2.4.1.1 additional information
-
pI: 5.5, fast isozyme Solanum tuberosum
2.4.1.1 5.2 5.6
-
Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 4 8 approx. half-maximal activity at pH 4 and pH 8, slow isozyme Solanum tuberosum
2.4.1.1 4.2 7.6 approx. half-maximal activity at pH 4.2 and 7.6, fast isozyme Solanum tuberosum