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Literature summary extracted from

  • Conrads, J.; Van Berkel, J.; Schächtele, C.; Steup, M.
    Non-chloroplast alpha-1,4-glucan phosphorylase from pea leaves: characterization and in situ localization by indirect immunofluorescence (1986), Biochim. Biophys. Acta, 882, 452-463.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast
-
 Pisum sativum 9507
-
2.4.1.1 cytoplasm predominant form, mesophyll and guard cells Pisum sativum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 88700
-
 2 * 88700, cytoplasmic isozyme, SDS-PAGE Pisum sativum
2.4.1.1 160000
-
 cytoplasmic isozyme, PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Pisum sativum
-
 var. kleine Rheinländerin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 2 of 3 isozymes: cytoplasmic I and major chloroplastic III form Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 11.7
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 -20°C, cytoplasmic isozyme, 12 months, approx. 30% loss of activity Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltodextrin)n-1 + glucose 1-phosphate maltodextrin with up to 11 glucose units Pisum sativum (maltodextrin)n + phosphate
-
 r
2.4.1.1 maltoheptaose + glucose 1-phosphate poor substrate Pisum sativum maltooctaose + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate best substrate Pisum sativum starch + phosphate
-
 ?
2.4.1.1 starch + glucose 1-phosphate soluble starch Pisum sativum starch + phosphate
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Pisum sativum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 88700, cytoplasmic isozyme, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 7 7.4 phosphorolysis Pisum sativum