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Literature summary extracted from

  • Mateyka, C.; Schnarrenberger, C.
    Purification and properties of mesophyll and bundle sheath cell alpha-glucan phosphorylases from Zea mays L. (1988), Plant Physiol., 86, 417-422.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Zea mays
2.4.1.1 1.5
-
 phosphate phosphorylase II Zea mays
2.4.1.1 1.9
-
 phosphate phosphorylase I Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast
-
 Spinacia oleracea 9507
-
2.4.1.1 chloroplast bundle sheath cell isozyme II Zea mays 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 53000
-
 4 * 53000, phosphorylase II, SDS-PAGE Zea mays
2.4.1.1 87000
-
 2 * 87000, phosphorylase I, SDS-PAGE Zea mays
2.4.1.1 174000
-
 phosphorylase I, ultracentrifugation Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Spinacia oleracea
-
-
-
2.4.1.1 Zea mays
-
 Inrafrüh
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 2 isozymes: I and II Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 leaf
-
 Spinacia oleracea
-
2.4.1.1 leaf mesophyll and bundle sheath, not epidermal cells Zea mays
-
2.4.1.1 mesophyll cell isozyme I Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 5
-
 phosphorylase II Zea mays
2.4.1.1 21
-
 phosphorylase I Zea mays
2.4.1.1 659
-
 phosphorylase I Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + glucose 1-phosphate
-
 Spinacia oleracea amylopectin + phosphate
-
 r
2.4.1.1 amylopectin + glucose 1-phosphate
-
 Zea mays amylopectin + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate
-
 Spinacia oleracea glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate
-
 Zea mays glycogen + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate
-
 Spinacia oleracea maltohexaose + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate
-
 Zea mays maltohexaose + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate soluble starch Zea mays starch + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Spinacia oleracea [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Zea mays [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer
-
 Spinacia oleracea
2.4.1.1 dimer 2 * 87000, phosphorylase I, SDS-PAGE Zea mays
2.4.1.1 tetramer 4 * 53000, phosphorylase II, SDS-PAGE Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.5 9
-
 Zea mays
2.4.1.1 5.7 8 approx. half-maximal activity at pH 5.7 and 8 Zea mays