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Literature summary extracted from
- Purification of a non-chloroplastic alpha-glucan phosphorylase from spinach leaves (1980), Planta, 148, 168-173.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.1 | freezing inactivates completely | Spinacia oleracea |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.1 | cytoplasm | - |
Spinacia oleracea | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 89000 | - |
2 * 89000, SDS-PAGE | Spinacia oleracea |
| 2.4.1.1 | 151000 | - |
gel filtration | Spinacia oleracea |
| 2.4.1.1 | 159000 | - |
porosity density gradient electrophoresis | Spinacia oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.1 | Spinacia oleracea | - |
spinach, var. Früremona or Kasperik | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.1 | non-chloroplastic phosphorylase I, ammonium sulfate, dextrin-Sepharose | Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.1 | leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 15.9 | - |
- |
Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.1 | 4°C, 2 weeks, 10% loss of activity | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Spinacia oleracea | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.1 | dimer | 2 * 89000, SDS-PAGE | Spinacia oleracea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.1 | pyridoxal 5'-phosphate | requirement | Spinacia oleracea | |
| 2.4.1.1 | pyridoxal 5'-phosphate | 1 mol/mol subunit | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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